PMID- 21154881
OWN - NLM
STAT- MEDLINE
DCOM- 20120222
LR  - 20131121
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 31
IP  - 7
DP  - 2011 Oct
TI  - Fluoride-induced genotoxicity in mouse bone marrow cells: effect of buthionine 
      sulfoximine and N-acetyl-L-cysteine.
PG  - 618-25
LID - 10.1002/jat.1605 [doi]
AB  - A significant level of reactive oxygen species generation was observed in sodium 
      fluoride (NaF) treated mouse bone marrow cells (BMCs). Reduced glutathione (GSH) 
      as a free radical scavenger could be an important determining factor in F-induced 
      genotoxicity. We therefore attempted to monitor GSH to understand the mechanism 
      of NaF-induced genotoxicity. NaF was injected intra-peritoneally in normal, 
      buthionine sulfoximine (BSO) or N-acetyl-L-cysteine (NAC) treated mice (n = 5). 
      After 13 h of NaF-treatment BMCs were collected to harvest them at the same 
      divisional cycle and processed for analysis of cell cycle, induction of apoptosis 
      and chromosomal aberrations (CAs). Level of GSH was also measured concomitantly. 
      NaF induced significant CAs in all treatment groups except at 2.5 mg NaF kg(-1) 
      body weight. BSO-treatment alone induced significantly high frequency of CAs. BSO 
      treatment prior to injection of 2.5-7.5 mg NaF kg(-1) b.w. was found to increase 
      the frequency of CAs, significantly when compared with the positive control 
      group, but the level was not significant in case of higher doses of NaF treatment 
      (15 and 30 mg kg(-1) b.w.). NaF-treated cells also showed a higher population of 
      Annexin-V positive cells. NAC pre-treatment significantly reduced the extent of 
      NaF-induced CAs, which clearly indicates the involvement of GSH in the NaF 
      response. However, further study is warranted to evaluate the low synergistic 
      effect of BSO on higher doses of NaF-induced CAs.
CI  - Copyright (c) 2010 John Wiley & Sons, Ltd.
FAU - Podder, Santosh
AU  - Podder S
AD  - Radiation Genetics and Chemical Mutagenesis Laboratory, Department of Zoology, 
      Centre for Advanced Studies, Visva-Bharati University, Santiniketan-731235, West 
      Bengal, India.
FAU - Chattopadhyay, Ansuman
AU  - Chattopadhyay A
FAU - Bhattacharya, Shelley
AU  - Bhattacharya S
FAU - Ray, Manas Ranjan
AU  - Ray MR
FAU - Chakraborty, Anindita
AU  - Chakraborty A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101210
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Annexin A5)
RN  - 0 (Reactive Oxygen Species)
RN  - 5072-26-4 (Buthionine Sulfoximine)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - GAN16C9B8O (Glutathione)
RN  - WYQ7N0BPYC (Acetylcysteine)
SB  - IM
MH  - Acetylcysteine/*pharmacology
MH  - Animals
MH  - Annexin A5/metabolism
MH  - Apoptosis/drug effects
MH  - Bone Marrow Cells/chemistry/*drug effects
MH  - Buthionine Sulfoximine/*pharmacology
MH  - Cell Cycle/drug effects
MH  - Chromosome Aberrations/drug effects
MH  - DNA Damage/*drug effects
MH  - Dose-Response Relationship, Drug
MH  - Glutathione/analysis
MH  - Male
MH  - Mice
MH  - Reactive Oxygen Species/metabolism
MH  - Sodium Fluoride/*toxicity
EDAT- 2010/12/15 06:00
MHDA- 2012/02/23 06:00
CRDT- 2010/12/15 06:00
PHST- 2010/07/01 00:00 [received]
PHST- 2010/08/02 00:00 [revised]
PHST- 2010/09/01 00:00 [accepted]
PHST- 2010/12/15 06:00 [entrez]
PHST- 2010/12/15 06:00 [pubmed]
PHST- 2012/02/23 06:00 [medline]
AID - 10.1002/jat.1605 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2011 Oct;31(7):618-25. doi: 10.1002/jat.1605. Epub 2010 Dec 10.