PMID- 21155720
OWN - NLM
STAT- MEDLINE
DCOM- 20110526
LR  - 20101215
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 77
IP  - 1
DP  - 2011 Jan
TI  - Regulation of HLA class II expression prevents allogeneic T-cell responses.
PG  - 36-44
LID - 10.1111/j.1399-0039.2010.01576.x [doi]
AB  - Human leukocyte antigen (HLA) class II molecules are polymorphic heterodimers 
      that present peptides to CD4+ T-cells. The HLA-DM molecule contributes to 
      assemble HLA class II-peptide complexes. We investigated the effect of silencing 
      either HLA-DR or HLA-DM expression in the allogeneic T-cell responses. The 
      delivery of HLA-DR- or HLA-DM-specific short hairpin RNAs (shRNAs) in a monocytic 
      cell line caused a decrease by up to 85% and 75% at the respective mRNA level. 
      Allogeneic T-cells stimulated with HLA-DM-silenced monocytes decreased to 30% 
      granzyme B mRNA and interferon gamma (IFN-gamma) production in comparison with 
      T-cells stimulated with monocytes expressing a non-specific shRNA. By contrast, 
      HLA-DR-silenced monocytes did not induce proliferation, up-regulation of granzyme 
      B mRNA levels or high IFN-gamma secretion by allogeneic T-cells vs HLA-DR expressing 
      cells. Direct targeting of HLA-DR expression prevented more efficiently an 
      allogeneic T-cell response in comparison with the knockdown of the expression of 
      HLA-DM molecules. Silencing the expression of HLA-DR molecules might contribute 
      to the development of new allogeneic cell-based therapeutic approaches.
CI  - (c) 2010 John Wiley & Sons A/S.
FAU - Jaimes, Y
AU  - Jaimes Y
AD  - Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany.
FAU - Seltsam, A
AU  - Seltsam A
FAU - Eiz-Vesper, B
AU  - Eiz-Vesper B
FAU - Blasczyk, R
AU  - Blasczyk R
FAU - Figueiredo, C
AU  - Figueiredo C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-D Antigens)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Flow Cytometry
MH  - *Gene Expression Regulation
MH  - Gene Silencing
MH  - HLA-D Antigens/genetics/*immunology
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - T-Lymphocytes/cytology/*immunology
EDAT- 2010/12/16 06:00
MHDA- 2011/05/27 06:00
CRDT- 2010/12/16 06:00
PHST- 2010/12/16 06:00 [entrez]
PHST- 2010/12/16 06:00 [pubmed]
PHST- 2011/05/27 06:00 [medline]
AID - 10.1111/j.1399-0039.2010.01576.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2011 Jan;77(1):36-44. doi: 10.1111/j.1399-0039.2010.01576.x.