PMID- 21155804
OWN - NLM
STAT- MEDLINE
DCOM- 20110621
LR  - 20211020
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Print)
IS  - 0022-3042 (Linking)
VI  - 117
IP  - 4
DP  - 2011 May
TI  - Epoxyeicosatrienoic acids enhance axonal growth in primary sensory and cortical 
      neuronal cell cultures.
PG  - 632-42
LID - 10.1111/j.1471-4159.2010.07139.x [doi]
AB  - It has recently been reported that soluble epoxide hydrolase (sEH), the major 
      enzyme that metabolizes epoxyeicosatrienoic acids (EETs), is expressed in axons 
      of cortical neurons; however, the functional relevance of axonal sEH localization 
      is unknown. Immunocytochemical analyses demonstrate predominant axonal 
      localization of sEH in primary cultures of not only cortical but also sympathetic 
      and sensory neurons. Morphometric analyses of cultured sensory neurons indicate 
      that exposure to a regioisomeric mixture of EETs (0.01-1.0 muM) causes a 
      concentration-dependent increase in axon outgrowth. This axon promoting activity 
      is not a generalized property of all regioisomers of EETs as axonal growth is 
      enhanced in sensory neurons exposed to 14,15-EET but not 8,9- or 11,12-EET. 
      14,15-EET also promotes axon outgrowth in cultured cortical neurons. Co-exposure 
      to EETs and either of two structurally diverse pharmacological inhibitors of sEH 
      potentiates the axon-enhancing activity of EETs in sensory and cortical neurons. 
      Mass spectrometry indicates that sEH inhibition significantly increases EETs and 
      significantly decreases dihydroxyeicosatrienoic acid metabolites in neuronal cell 
      cultures. These data indicate that EETs enhance axon outgrowth and suggest that 
      axonal sEH activity regulates EETs-induced axon outgrowth. These findings suggest 
      a novel therapeutic use of sEH inhibitors in promoting nerve regeneration.
CI  - (c) 2011 The Authors. Journal of Neurochemistry (c) 2011 International Society for 
      Neurochemistry.
FAU - Abdu, Emun
AU  - Abdu E
AD  - Department of Neurological Surgery, Oregon Health & Science University, Portland, 
      Oregon, USA.
FAU - Bruun, Donald A
AU  - Bruun DA
FAU - Yang, Dongren
AU  - Yang D
FAU - Yang, Jun
AU  - Yang J
FAU - Inceoglu, Bora
AU  - Inceoglu B
FAU - Hammock, Bruce D
AU  - Hammock BD
FAU - Alkayed, Nabil J
AU  - Alkayed NJ
FAU - Lein, Pamela J
AU  - Lein PJ
LA  - eng
GR  - NS044313/NS/NINDS NIH HHS/United States
GR  - P42ES004699/ES/NIEHS NIH HHS/United States
GR  - ES014901/ES/NIEHS NIH HHS/United States
GR  - R01 ES002710/ES/NIEHS NIH HHS/United States
GR  - P42 ES004699/ES/NIEHS NIH HHS/United States
GR  - R01 ES002710-31/ES/NIEHS NIH HHS/United States
GR  - R01 NS046649-05/NS/NINDS NIH HHS/United States
GR  - R01 ES014901/ES/NIEHS NIH HHS/United States
GR  - P30 NS061800/NS/NINDS NIH HHS/United States
GR  - R01 NS044313/NS/NINDS NIH HHS/United States
GR  - R56 ES014901/ES/NIEHS NIH HHS/United States
GR  - ES002710/ES/NIEHS NIH HHS/United States
GR  - R01 NS044313-01/NS/NINDS NIH HHS/United States
GR  - R01 NS046649/NS/NINDS NIH HHS/United States
GR  - NS046649/NS/NINDS NIH HHS/United States
GR  - R01 NS070837/NS/NINDS NIH HHS/United States
GR  - P42 ES004699-23S2/ES/NIEHS NIH HHS/United States
GR  - R01 ES014901-02/ES/NIEHS NIH HHS/United States
GR  - R37 ES002710/ES/NIEHS NIH HHS/United States
GR  - R01 ES002710-30/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110124
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Eicosanoic Acids)
RN  - EC 3.3.2.- (Epoxide Hydrolases)
SB  - IM
MH  - Animals
MH  - Axons/*physiology
MH  - Cell Count
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/drug effects/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Eicosanoic Acids/*pharmacology
MH  - Epoxide Hydrolases/metabolism
MH  - Female
MH  - Ganglia, Spinal/cytology/drug effects
MH  - Immunohistochemistry
MH  - Neurons/drug effects
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sensory Receptor Cells/drug effects/*physiology
MH  - Subcellular Fractions/metabolism
MH  - Sympathetic Nervous System/cytology/drug effects
PMC - PMC3081369
MID - NIHMS257940
EDAT- 2010/12/16 06:00
MHDA- 2011/06/22 06:00
PMCR- 2012/05/01
CRDT- 2010/12/16 06:00
PHST- 2010/12/16 06:00 [entrez]
PHST- 2010/12/16 06:00 [pubmed]
PHST- 2011/06/22 06:00 [medline]
PHST- 2012/05/01 00:00 [pmc-release]
AID - 10.1111/j.1471-4159.2010.07139.x [doi]
PST - ppublish
SO  - J Neurochem. 2011 May;117(4):632-42. doi: 10.1111/j.1471-4159.2010.07139.x. Epub 
      2011 Jan 24.