PMID- 21156226
OWN - NLM
STAT- MEDLINE
DCOM- 20110104
LR  - 20211020
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 203
IP  - 2
DP  - 2010 Dec
TI  - Standardization of fluorescence in situ hybridization studies on chronic 
      lymphocytic leukemia (CLL) blood and marrow cells by the CLL Research Consortium.
PG  - 141-8
LID - 10.1016/j.cancergencyto.2010.08.009 [doi]
AB  - Five laboratories in the Chronic Lymphocytic Leukemia (CLL) Research Consortium 
      (CRC) investigated standardizing and pooling of fluorescence in situ 
      hybridization (FISH) results as a collaborative research project. This 
      investigation used fixed bone marrow and blood cells available from previous 
      conventional cytogenetic or FISH studies in two pilot studies, a one-day 
      workshop, and proficiency test. Multiple FISH probe strategies were used to 
      detect 6q-, 11q-, +12, 13q-, 17p-, and IGH rearrangements. Ten specimens were 
      studied by participants who used their own probes (pilot study 1). Of 312 FISH 
      interpretations, 224 (72%) were true-negative, 74 (24%) true-positive, 6 (2%) 
      false-negative, and 8 (3%) false-positive. In pilot study no. 2, each participant 
      studied two specimens using identical FISH probe sets to control for variation 
      due to probe sets and probe strategies. Of 80 FISH interpretations, no false 
      interpretations were identified. At a subsequent workshop, discussions produced 
      agreement on scoring criteria. The proficiency test that followed produced no 
      false-negative results and 4% (3/68) false-positive interpretations. 
      Interpretation disagreements among laboratories were primarily attributable to 
      inadequate normal cutoffs, inconsistent scoring criteria, and the use of 
      different FISH probe strategies. Collaborative organizations that use pooled FISH 
      results may wish to impose more conservative empiric normal cutoff values or use 
      an equivocal range between the normal cutoff and the abnormal reference range to 
      eliminate false-positive interpretations. False-negative results will still 
      occur, and would be expected in low-percentage positive cases; these would likely 
      have less clinical significance than false positive results. Individual 
      laboratories can help by closely following rigorous quality assurance guidelines 
      to ensure accurate and consistent FISH studies in their clinical practice and 
      research.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Smoley, Stephanie A
AU  - Smoley SA
AD  - Cytogenetics, Division of Laboratory Genetics, Department of Laboratory Medicine, 
      200 First Street SW, Rochester, MN 55905, USA.
FAU - Van Dyke, Daniel L
AU  - Van Dyke DL
FAU - Kay, Neil E
AU  - Kay NE
FAU - Heerema, Nyla A
AU  - Heerema NA
FAU - Dell' Aquila, Marie L
AU  - Dell' Aquila ML
FAU - Dal Cin, Paola
AU  - Dal Cin P
FAU - Koduru, Prasad
AU  - Koduru P
FAU - Aviram, Ayala
AU  - Aviram A
FAU - Rassenti, Laura
AU  - Rassenti L
FAU - Byrd, John C
AU  - Byrd JC
FAU - Rai, Kanti R
AU  - Rai KR
FAU - Brown, Jennifer R
AU  - Brown JR
FAU - Greaves, Andrew W
AU  - Greaves AW
FAU - Eckel-Passow, Jeanette
AU  - Eckel-Passow J
FAU - Neuberg, Donna
AU  - Neuberg D
FAU - Kipps, Thomas J
AU  - Kipps TJ
FAU - Dewald, Gordon W
AU  - Dewald GW
LA  - eng
GR  - P01 CA081534/CA/NCI NIH HHS/United States
GR  - R01 CA095241/CA/NCI NIH HHS/United States
GR  - P01-CA81534/CA/NCI NIH HHS/United States
GR  - CA95241/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Bone Marrow Cells/cytology
MH  - Cytogenetics/*standards
MH  - False Negative Reactions
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Microscopy, Fluorescence/methods
MH  - Oligonucleotide Probes/genetics
MH  - Pilot Projects
MH  - Reproducibility of Results
PMC - PMC3763815
MID - NIHMS493106
EDAT- 2010/12/16 06:00
MHDA- 2011/01/05 06:00
PMCR- 2013/09/05
CRDT- 2010/12/16 06:00
PHST- 2010/05/09 00:00 [received]
PHST- 2010/07/13 00:00 [revised]
PHST- 2010/08/05 00:00 [accepted]
PHST- 2010/12/16 06:00 [entrez]
PHST- 2010/12/16 06:00 [pubmed]
PHST- 2011/01/05 06:00 [medline]
PHST- 2013/09/05 00:00 [pmc-release]
AID - S0165-4608(10)00451-6 [pii]
AID - 10.1016/j.cancergencyto.2010.08.009 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2010 Dec;203(2):141-8. doi: 
      10.1016/j.cancergencyto.2010.08.009.