PMID- 21156229
OWN - NLM
STAT- MEDLINE
DCOM- 20110104
LR  - 20101215
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 203
IP  - 2
DP  - 2010 Dec
TI  - Correlation of cytomorphology, immunophenotyping, and interphase fluorescence in 
      situ hybridization in 381 patients with monoclonal gammopathy of undetermined 
      significance and 301 patients with plasma cell myeloma.
PG  - 169-75
LID - 10.1016/j.cancergencyto.2010.08.006 [doi]
AB  - To further clarify the transformation from monoclonal gammopathy of undetermined 
      significance (MGUS) to plasma cell myeloma (PCM), we compared interphase 
      fluorescence in situ hybridization (FISH) patterns in 381 MGUS and 301 PCM 
      patients. According to the World Health Organization and the International 
      Myeloma Working Group, a threshold of 10% of bone marrow plasma cells separated 
      MGUS from PCM. After magnetic activated cell sorting for CD138(+) cells, FISH 
      succeeded in 272 of 301 (90.4%) PCM, but in only 302 of 381 (79.3%) MGUS cases (P 
      < 0.001). Cytogenetic alterations were more frequent in PCM (237 of 272; 87.1%) 
      than MGUS (169 of 302; 56.0%; P = 0.0002). PCM showed a median of two cytogenetic 
      alterations (range, 0-9) and MGUS one (range, 0-6). Considering only cases with a 
      yield of plasma cells allowing five or more FISH probes, del(13)(q14) was found 
      in 99 of 251 (39.3%) PCM but in only 59 of 267 (22.1%) MGUS (P = 0.0001), 
      del(17p) in 15 PCM (6.0%) and in 6 MGUS (2.2%) patients (P = 0.029). A 
      t(4;14)/IGH-FGFR3 was detected in 28 PCM (11.1%) and 5 MGUS (1.9%; P < 0.001). 
      The t(11;14)/IGH-CCND1 and the t(14;16)/IGH-MAF showed no significant 
      differences. Cytomorphology detected higher numbers of plasma cells than 
      multiparameter flow cytometry (median ratio 4.25). This study underlines the 
      genetic heterogeneity of MGUS similar to PCM. Genetic analysis might contribute 
      to more diversified monitoring strategies for MGUS patients.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Bacher, Ulrike
AU  - Bacher U
AD  - Clinic for Stem Cell Transplantation, University Cancer Center Hamburg, 
      Martinistr 52, 20246 Hamburg, Germany. u.bacher@uke.de
FAU - Haferlach, Torsten
AU  - Haferlach T
FAU - Kern, Wolfgang
AU  - Kern W
FAU - Alpermann, Tamara
AU  - Alpermann T
FAU - Schnittger, Susanne
AU  - Schnittger S
FAU - Haferlach, Claudia
AU  - Haferlach C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (Syndecan-1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Separation
MH  - Chromosome Deletion
MH  - Cytogenetic Analysis
MH  - Cytogenetics
MH  - Female
MH  - Flow Cytometry/methods
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Models, Genetic
MH  - Multiple Myeloma/*genetics/*immunology
MH  - Paraproteinemias/*genetics/*immunology
MH  - Syndecan-1/biosynthesis
EDAT- 2010/12/16 06:00
MHDA- 2011/01/05 06:00
CRDT- 2010/12/16 06:00
PHST- 2010/02/19 00:00 [received]
PHST- 2010/06/18 00:00 [revised]
PHST- 2010/08/01 00:00 [accepted]
PHST- 2010/12/16 06:00 [entrez]
PHST- 2010/12/16 06:00 [pubmed]
PHST- 2011/01/05 06:00 [medline]
AID - S0165-4608(10)00447-4 [pii]
AID - 10.1016/j.cancergencyto.2010.08.006 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2010 Dec;203(2):169-75. doi: 
      10.1016/j.cancergencyto.2010.08.006.