PMID- 21156239
OWN - NLM
STAT- MEDLINE
DCOM- 20110104
LR  - 20101215
IS  - 1873-4456 (Electronic)
IS  - 0165-4608 (Linking)
VI  - 203
IP  - 2
DP  - 2010 Dec
TI  - Evaluation of upper urinary tract tumors by FISH in Chinese patients.
PG  - 238-46
LID - 10.1016/j.cancergencyto.2010.07.133 [doi]
AB  - Upper urinary tract tumor (UUTT) usually presents a high grade and stage, and 
      recurs frequently. The aim of this study was to evaluate the utility of a 
      fluorescence in situ hybridization (FISH) assay on chromosomes 3, 7, 9, and 17 as 
      a reliable and noninvasive method for the diagnosis of Chinese patients with 
      UUTT. Urine specimens from 50 patients with UUTT and 25 donors without evidence 
      of urothelial tumors were analyzed by cytology and FISH. Voided urine samples 
      from 20 normal individuals were used to establish the cut-off values for FISH 
      assay. The McNemar test was applied for sensitivity and specificity. The overall 
      sensitivity of FISH was statistically significantly greater than that of cytology 
      (84.0 vs. 40.0%, P = 0.000). The overall specificities of FISH and urine cytology 
      were all 96.0% (P = 1.000). Polysomy in chromosomes 3, 7, and 17 were 38, 42, and 
      30%, respectively. Heterozygous and homozygous loss of the p16 locus was found in 
      36 and 32%, respectively. FISH analysis performed on cells collected from voided 
      urine is feasible, and FISH could prove to be a reliable and less invasive 
      ancillary test and improve the sensitivity of urine cytology in the diagnosis of 
      UUTT.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Shan, Zhengfei
AU  - Shan Z
AD  - Department of Urology, Nan fang Hospital, Southern Medical University, No. 1838 
      North Guangzhou avenue, Guangzhou 510515, People's Republic of China.
FAU - Wu, Peng
AU  - Wu P
FAU - Zheng, Shaobin
AU  - Zheng S
FAU - Tan, Wanlong
AU  - Tan W
FAU - Zhou, Haikuan
AU  - Zhou H
FAU - Zuo, Yi
AU  - Zuo Y
FAU - Qi, Huan
AU  - Qi H
FAU - Zhang, Peng
AU  - Zhang P
FAU - Peng, Hongmei
AU  - Peng H
FAU - Wang, Yanfen
AU  - Wang Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Centromere/ultrastructure
MH  - China
MH  - Cytological Techniques
MH  - Female
MH  - Gene Deletion
MH  - Heterozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - Urologic Neoplasms/*genetics
EDAT- 2010/12/16 06:00
MHDA- 2011/01/05 06:00
CRDT- 2010/12/16 06:00
PHST- 2010/04/14 00:00 [received]
PHST- 2010/07/12 00:00 [revised]
PHST- 2010/07/25 00:00 [accepted]
PHST- 2010/12/16 06:00 [entrez]
PHST- 2010/12/16 06:00 [pubmed]
PHST- 2011/01/05 06:00 [medline]
AID - S0165-4608(10)00437-1 [pii]
AID - 10.1016/j.cancergencyto.2010.07.133 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2010 Dec;203(2):238-46. doi: 
      10.1016/j.cancergencyto.2010.07.133.