PMID- 21158076
OWN - NLM
STAT- MEDLINE
DCOM- 20120531
LR  - 20131121
IS  - 1000-6834 (Print)
IS  - 1000-6834 (Linking)
VI  - 22
IP  - 3
DP  - 2006 Aug
TI  - [Interleukin-6 prevents cultured cerebellar granule neurons from 
      glutamate-induced neurotoxicity].
PG  - 310-5
AB  - AIM: To explore IL-6 neuroprotection against glutamate-induced neurotoxicity and 
      primary mechanisms involved in this neuroprotection. METHODS: The cerebellar 
      granule neurons from postnatal 8-day infant rats were chronically exposed to IL-6 
      for 8 days, and then glutamate stimulated the cultured cerebellar granule neurons 
      for 15 min. Methyl-thiazole-tetrazolium (MTT) assay and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) method were used to observe 
      the changes of neuronal vitality and apoptosis, respectively. Laser scanning 
      confocal microscope (LSCM) and reverse transcription-polymerase chain reaction 
      (RT-PCR) were respectively employed to measure dynamic changes of intracellular 
      Ca2+ levels and expression of gp130 mRNA, a 130-kDa intracellular IL-6 
      signal-transduction protein, in the neurons. RESULTS: The chronic IL-6 (2.5, 5 
      and 10 ng/ml) pretreatment of the cultured cerebellar granule neurons remarkably 
      improved the decreased neuronal vitality by glutamate in a 
      concentration-dependent manner. The neuronal apoptosis induced by glutamate was 
      significantly attenuated by the chronic IL-6 pretreatment. The intracellular Ca2+ 
      overload evoked by glutamate was also inhibited by the chronic IL-6 pretreatment. 
      The expression of gp130 mRNA was dramatically lower in the IL-6-pretreated 
      cerebellar granule neurons than in the IL-6-untreated neurons. CONCLUSION: IL-6 
      can protect neurons against glutamate-induced exciting neurotoxicity. The 
      mechanism of IL-6 neuroprotection may be closely related to the suppression of 
      glutamate-induced intracellular Ca2+ overload and mediated by gp130 intracellular 
      signal transduction pathways.
FAU - Lu, Jian-hua
AU  - Lu JH
AD  - Department of Physiology, School of Basic Medical Sciences, Nantong University, 
      Nantong 226001, China.
FAU - Qiu, Yi-hua
AU  - Qiu YH
FAU - Peng, Yu-ping
AU  - Peng YP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Ying Yong Sheng Li Xue Za Zhi
JT  - Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = 
      Chinese journal of applied physiology
JID - 9426407
RN  - 0 (Interleukin-6)
RN  - 0 (Neuroprotective Agents)
RN  - 3KX376GY7L (Glutamic Acid)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cerebellum/cytology/*drug effects/metabolism
MH  - Glutamic Acid/*toxicity
MH  - Interleukin-6/*pharmacology
MH  - Neurons/*drug effects/metabolism
MH  - Neuroprotective Agents/*pharmacology
MH  - Neurotoxicity Syndromes/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2006/08/01 00:00
MHDA- 2012/06/01 06:00
CRDT- 2010/12/17 06:00
PHST- 2010/12/17 06:00 [entrez]
PHST- 2006/08/01 00:00 [pubmed]
PHST- 2012/06/01 06:00 [medline]
PST - ppublish
SO  - Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2006 Aug;22(3):310-5.