PMID- 21158944
OWN - NLM
STAT- MEDLINE
DCOM- 20110321
LR  - 20101216
IS  - 1399-3089 (Electronic)
IS  - 0908-665X (Linking)
VI  - 17
IP  - 6
DP  - 2010 Nov-Dec
TI  - Long-term absence of porcine endogenous retrovirus infection in chronically 
      immunosuppressed patients after treatment with the porcine cell-based Academic 
      Medical Center bioartificial liver.
PG  - 431-9
LID - 10.1111/j.1399-3089.2010.00617.x [doi]
AB  - BACKGROUND: Clinical use of porcine cell-based bioartificial liver (BAL) support 
      in acute liver failure as bridging therapy for liver transplantation exposes the 
      patient to the risk of transmission of porcine endogenous retroviruses (PERVs) to 
      human. This risk may be enhanced when patients receive liver transplant and are 
      subsequently immunosuppressed. As further follow-up of previously reported 
      patients (Di Nicuolo et al. 2005), an assessment of PERV infection was made in 
      the same patient population pharmacologically immunosuppressed for several years 
      after BAL treatment and in healthcare workers (HCWs) involved in the clinical 
      trial at that time. METHODS: Plasma and peripheral blood mononuclear cells 
      (PBMCs) from eight patients treated with the Academic Medical Center-BAL 
      (AMC-BAL), who survived to transplant, and 13 HCWs, who were involved in the 
      trial, were assessed to detect PERV infection. A novel quantitative real-time 
      polymerase chain reaction assay has been used. RESULTS: Eight patients who 
      received a liver transplant after AMC-BAL treatment are still alive under 
      long-term pharmacological immunosuppression. The current clinical follow-up 
      ranges from 5.6 to 8.7 yr after BAL treatment. A new q-real-time PCR assay has 
      been developed and validated to detect PERV infection. The limit of 
      quantification of PERV DNA was >/= 5 copies per 1 x 10(5) PBMCs. The linear dynamic 
      range was from 5 x 10(0) to 5 x 10(6) copies. In both patients and HCWs, neither 
      PERV DNA in PBMCs nor PERV RNA in plasma and PBMC samples have been found. 
      CONCLUSION: Up to 8.7 yr after exposure to treatment with porcine liver 
      cell-based BAL, no PERV infection has been found in long-term immunosuppressed 
      patients and in HCWs by a new highly sensitive and specific q-real-time PCR 
      assay.
CI  - (c) 2010 John Wiley & Sons A/S.
FAU - Di Nicuolo, Giuseppe
AU  - Di Nicuolo G
AD  - Laboratory of Virology, Cotugno Hospital, Naples, Italy.
FAU - D'Alessandro, Alba
AU  - D'Alessandro A
FAU - Andria, Barbara
AU  - Andria B
FAU - Scuderi, Vincenzo
AU  - Scuderi V
FAU - Scognamiglio, Michele
AU  - Scognamiglio M
FAU - Tammaro, Angela
AU  - Tammaro A
FAU - Mancini, Antonio
AU  - Mancini A
FAU - Cozzolino, Santolo
AU  - Cozzolino S
FAU - Di Florio, Ernesto
AU  - Di Florio E
FAU - Bracco, Adele
AU  - Bracco A
FAU - Calise, Fulvio
AU  - Calise F
FAU - Chamuleau, Robert A F M
AU  - Chamuleau RA
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Xenotransplantation
JT  - Xenotransplantation
JID - 9438793
RN  - 0 (DNA, Viral)
RN  - 0 (Immunosuppressive Agents)
SB  - IM
CIN - Xenotransplantation. 2010 Nov-Dec;17(6):429-30. PMID: 21158943
MH  - Animals
MH  - DNA, Viral/blood
MH  - Endogenous Retroviruses/genetics/*pathogenicity
MH  - Humans
MH  - *Immunocompromised Host
MH  - Immunosuppressive Agents/therapeutic use
MH  - Liver Transplantation/adverse effects/immunology
MH  - Liver, Artificial/*virology
MH  - Retroviridae Infections/*etiology
MH  - Swine
MH  - Transplantation, Heterologous/*adverse effects/immunology
EDAT- 2010/12/17 06:00
MHDA- 2011/03/22 06:00
CRDT- 2010/12/17 06:00
PHST- 2010/12/17 06:00 [entrez]
PHST- 2010/12/17 06:00 [pubmed]
PHST- 2011/03/22 06:00 [medline]
AID - 10.1111/j.1399-3089.2010.00617.x [doi]
PST - ppublish
SO  - Xenotransplantation. 2010 Nov-Dec;17(6):431-9. doi: 
      10.1111/j.1399-3089.2010.00617.x.