PMID- 21159361
OWN - NLM
STAT- MEDLINE
DCOM- 20110411
LR  - 20171116
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 82
IP  - 11
DP  - 2011 Mar
TI  - Characterization of bacterial communities at heavy-metal-contaminated sites.
PG  - 1583-8
LID - 10.1016/j.chemosphere.2010.11.056 [doi]
AB  - The microbial community in soil samples from two long-term contaminated sites was 
      characterized by using culture-dependent and culture-independent methods. The two 
      sites investigated contained high amounts of heavy metals and were located in the 
      upper Silesia Industrial Region in southern Poland. The evaluation of the aerobic 
      soil microbial population clearly demonstrated the presence of considerable 
      numbers of viable, culturable bacteria at both sites. A high fraction of the 
      bacterial population was able to grow in the presence of high amounts of metals, 
      i.e. up to 10 mM Zn(2)(+), 3 mM Pb(2)(+) or 1 mM Cu(2)(+). Site 1 contained 
      significantly (P < 0.05) lower bacterial numbers growing in the presence of 10 mM 
      Zn(2)(+) than site 2, while the opposite was observed for bacteria tolerating 1 mM 
      Cu(2)(+). This coincided with the contents of these two metals at the two sites. 
      Ecophysiological (EP) indices for copiotrophs (r-strategists) and oligotrophs 
      (K-strategists) pointed to high bacterial diversity at both sites. Fluorescence 
      in situ hybridization (FISH) analysis indicated that Actinobacteria and 
      Proteobacteria represent the physiologically active fraction of bacteria at the 
      two sites. Shannon diversity (H') indices for FISH-detected bacterial 
      phylogenetic groups were not significantly different at the two sites.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Margesin, Rosa
AU  - Margesin R
AD  - Institute of Microbiology, University of Innsbruck, Innsbruck, Austria. 
      rosa.margesin@uibk.ac.at
FAU - Plaza, Grazyna A
AU  - Plaza GA
FAU - Kasenbacher, Stefanie
AU  - Kasenbacher S
LA  - eng
PT  - Journal Article
DEP - 20101214
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Metals, Heavy)
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
SB  - IM
MH  - Bacteria, Aerobic/*classification/drug effects/metabolism
MH  - Biodiversity
MH  - Colony Count, Microbial
MH  - Environmental Monitoring
MH  - In Situ Hybridization, Fluorescence
MH  - Metals, Heavy/analysis/metabolism/*toxicity
MH  - Soil/chemistry
MH  - Soil Microbiology
MH  - Soil Pollutants/analysis/metabolism/*toxicity
EDAT- 2010/12/17 06:00
MHDA- 2011/04/13 06:00
CRDT- 2010/12/17 06:00
PHST- 2010/08/30 00:00 [received]
PHST- 2010/11/07 00:00 [revised]
PHST- 2010/11/21 00:00 [accepted]
PHST- 2010/12/17 06:00 [entrez]
PHST- 2010/12/17 06:00 [pubmed]
PHST- 2011/04/13 06:00 [medline]
AID - S0045-6535(10)01363-9 [pii]
AID - 10.1016/j.chemosphere.2010.11.056 [doi]
PST - ppublish
SO  - Chemosphere. 2011 Mar;82(11):1583-8. doi: 10.1016/j.chemosphere.2010.11.056. Epub 
      2010 Dec 14.