PMID- 2116396
OWN - NLM
STAT- MEDLINE
DCOM- 19900910
LR  - 20240324
IS  - 0910-5050 (Print)
IS  - 1876-4673 (Electronic)
IS  - 0910-5050 (Linking)
VI  - 81
IP  - 5
DP  - 1990 May
TI  - Two-dimensional electrophoretic analysis of hepatitis-associated polypeptides in 
      liver of LEC rats developing spontaneous hepatitis.
PG  - 477-82
AB  - High-resolution two-dimensional polyacrylamide gel electrophoresis in combination 
      with silver staining was used to analyze between 800 and 1000 cytosolic and 
      particulate polypeptides from age-matched livers of normal male Long-Evans rat 
      with Agouti coat color (LEA) and Long-Evans rat with Cinnamon-like coat color 
      (LEC) rats with hereditary trait of hepatitis at ages long before, immediately 
      prior to, and just after the onset of hepatitis. Although the electrophoretic 
      patterns of polypeptide expression were very similar with respect to the overall 
      spot patterns, a number of polypeptides which differed either qualitatively or 
      quantitatively were noted. Two constitutively expressed cytosolic polypeptides, 
      P29.5 (Mr 29.5 kDa/pI 6.73) and P30 (30 kDa/6.70), were not detected in livers of 
      LEC animals at any age. In the normal LEA rats both P29.5 and P30 were detected 
      as early as one day after birth and both were expressed at similar concentrations 
      at all ages. In the LEC rats P30-C (30 kDa/6.68) was constitutively expressed in 
      close proximity to the expected position of P30, and P30-C was not detected in 
      the LEA rats. By means of non-equilibrium pH gradient electrophoresis two 
      relatively basic polypeptides were detected in the LEC rats. P18ne was detected 
      immediately prior to and P27ne immediately after the clinical manifestation of 
      hepatitis. Experiments in F1 backcross ([LEA x LEC] x LEC) animals, however, 
      failed to demonstrate any genetic link between either the expression or lack of 
      expression of P29.5, P30, P30-C, or P18ne and hepatitis development. P27ne was 
      detected in all backcross animals exhibiting hepatitis, but was never observed in 
      LEC rats prior to the onset of hepatitis. Although we were unable to identify any 
      unique loss of expression of polypeptides which are genetically linked to 
      hepatitis susceptibility in LEC rats, specific subsets of quantitatively 
      modulated polypeptides were detected.
FAU - Wirth, P J
AU  - Wirth PJ
AD  - Carcinogenesis Division, National Cancer Center Research Institute, Tokyo.
FAU - Fujimoto, Y
AU  - Fujimoto Y
FAU - Takahashi, H
AU  - Takahashi H
FAU - Mori, M
AU  - Mori M
FAU - Yoshida, M C
AU  - Yoshida MC
FAU - Sugimura, T
AU  - Sugimura T
FAU - Nagao, M
AU  - Nagao M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 0 (Peptides)
SB  - IM
MH  - Animals
MH  - Cytosol/analysis
MH  - Electrophoresis, Polyacrylamide Gel
MH  - *Hepatitis, Animal
MH  - Liver/*analysis
MH  - Peptides/*analysis
MH  - Rats
MH  - Rats, Inbred Strains
PMC - PMC5918070
EDAT- 1990/05/01 00:00
MHDA- 1990/05/01 00:01
PMCR- 1990/05/01
CRDT- 1990/05/01 00:00
PHST- 1990/05/01 00:00 [pubmed]
PHST- 1990/05/01 00:01 [medline]
PHST- 1990/05/01 00:00 [entrez]
PHST- 1990/05/01 00:00 [pmc-release]
AID - CAE477 [pii]
AID - 10.1111/j.1349-7006.1990.tb02594.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 1990 May;81(5):477-82. doi: 10.1111/j.1349-7006.1990.tb02594.x.