PMID- 21165643
OWN - NLM
STAT- MEDLINE
DCOM- 20111108
LR  - 20211020
IS  - 1439-6327 (Electronic)
IS  - 1439-6319 (Linking)
VI  - 111
IP  - 7
DP  - 2011 Jul
TI  - HGF inhibits TGF-beta1-induced myofibroblast differentiation and ECM deposition via 
      MMP-2 in Achilles tendon in rat.
PG  - 1457-63
LID - 10.1007/s00421-010-1764-4 [doi]
AB  - Both myofibroblast differentiation and extracellular matrix (ECM) deposition are 
      essential components of scar formation in tendons, and hepatocyte growth factor 
      (HGF) is reported to prevent fibrogenic responses in tendons. Matrix 
      metalloproteinases-2(MMP-2) is also involved in the healing process in tendons. 
      Whether HGF protects healed Achilles tendons from injury-induced scar formation 
      and the mechanisms are unknown. Daily for 2 weeks after wounding, except for the 
      non-surgical control group, the Achilles tendons in rats were locally injected 
      with HGF (100 ng 50 mul(-1) per mouse) or phosphate-buffered saline (PBS). 
      Histological examination showed HGF ameliorated disorganized collagen fibers 
      caused by surgical incisions in rats. After transforming growth factor beta-1 
      (TGF-beta1) induced fibrogenic responses in primary Achilles tendon fibroblasts in 
      rats, HGF treatment for 24 h reduced alpha-smooth muscle actin (alpha-SMA) (0.60 +/- 
      0.07-fold, P < 0.05) and type III collagen expression (0.39 +/- 0.07-fold, P < 
      0.05). Moreover, HGF elevated MMP-2 expression (1.23 +/- 0.11-fold, P < 0.05). The 
      MMP-2 inhibitor, tissue inhibitors of metalloproteinase-1 (TIMP-1), partially 
      blocked the inhibitory effects of HGF on alpha-SMA expression (from 0.60 +/- 0.07-fold 
      to 0.83 +/- 0.07-fold, P < 0.05) and type III collagen expression (from 0.39 +/- 
      0.06-fold to 0.86 +/- 0.08-fold, P < 0.05). These results indicate HGF attenuates 
      TGF-beta1-induced fibrogenic responses in Achilles tendon, which was mediated by 
      MMP-2. These results will aid in developing effective therapeutic approaches for 
      the dysfunctional repair in Achilles tendons.
FAU - Cui, Qingbo
AU  - Cui Q
AD  - Pediatric Orthopedics Unit, Second Affiliated Hospital of Harbin Medical 
      University, 246 Xuefu Road, Harbin 150086, China.
FAU - Wang, Zhigang
AU  - Wang Z
FAU - Jiang, Dapeng
AU  - Jiang D
FAU - Qu, Lihui
AU  - Qu L
FAU - Guo, Junbin
AU  - Guo J
FAU - Li, Zhaozhu
AU  - Li Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101217
PL  - Germany
TA  - Eur J Appl Physiol
JT  - European journal of applied physiology
JID - 100954790
RN  - 0 (Fibrillar Collagens)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
SB  - IM
MH  - Achilles Tendon/cytology/*drug effects/metabolism/physiology
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Drug Evaluation, Preclinical
MH  - Extracellular Matrix/*metabolism
MH  - Fibrillar Collagens/metabolism
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Injections
MH  - Male
MH  - Matrix Metalloproteinase 2/metabolism/*physiology
MH  - Mice
MH  - Myofibroblasts/*drug effects/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor beta1/*pharmacology
MH  - Up-Regulation/drug effects
EDAT- 2010/12/18 06:00
MHDA- 2011/11/09 06:00
CRDT- 2010/12/18 06:00
PHST- 2010/11/27 00:00 [accepted]
PHST- 2010/12/18 06:00 [entrez]
PHST- 2010/12/18 06:00 [pubmed]
PHST- 2011/11/09 06:00 [medline]
AID - 10.1007/s00421-010-1764-4 [doi]
PST - ppublish
SO  - Eur J Appl Physiol. 2011 Jul;111(7):1457-63. doi: 10.1007/s00421-010-1764-4. Epub 
      2010 Dec 17.