PMID- 21166311
OWN - NLM
STAT- MEDLINE
DCOM- 20110120
LR  - 20101220
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 92
IP  - 1
DP  - 2010 Oct 26
TI  - First isolation and genotyping of viruses from recent outbreaks of viral 
      haemorrhagic septicaemia (VHS) in Slovenia.
PG  - 21-9
LID - 10.3354/dao02251 [doi]
AB  - In November and December 2007, the virus causing viral haemorrhagic septicaemia 
      (VHS) was detected in rainbow trout Oncorhynchus mykiss from 2 fish farms in 
      Slovenia. During 2008 and 2009 the infection spread only among rainbow trout 
      farms and 4 new outbreaks were confirmed. High mortality and clinical signs of 
      VHS were observed among the diseased fish. VHSV was confirmed by virus isolation, 
      immunoperoxidase test, reverse transcriptase polymerase chain reaction (RT-PCR) 
      and phylogenetic analysis. Based on 1 complete (1524 nucleotides [nt]) and 9 
      partial (600 nt) glycoprotein gene nucleotide sequences, 9 VHSV isolates from the 
      6 VHS outbreaks were genetically closely related (99 to 100% identity), and were 
      classified into the Subgroup I-a of Genotype I, most closely related to the 
      German isolates Dstg21-07, Dstg36-06, and Dstg54-1-07 (99 to 100% identity). 
      Phylogenetic analysis and epidemiological investigations confirmed that the VHS 
      virus had been (re)introduced with imported live fish, and that subsequent 
      outbreaks were linked to the initial infection. Our study shows that direct 
      nucleotide sequencing of RT-PCR products, amplified from the tissue of 
      VHSV-infected fish, represents a reliable tool for fast routine genotyping in 
      diagnostic laboratories. This is the first report of a natural epidemic 
      associated with VHSV infection in Slovenia since the eradication of the disease 
      in 1977.
FAU - Toplak, Ivan
AU  - Toplak I
AD  - Virology Unit, Institute of Microbiology and Parasitology, Veterinary Faculty, 
      University of Ljubljana, Gerbiceva 60, 1115 Ljubljana, Slovenia. 
      ivan.toplak@vf.uni-lj.si
FAU - Hostnik, Peter
AU  - Hostnik P
FAU - Rihtaric, Danijela
AU  - Rihtaric D
FAU - Olesen, Niels Jorgen
AU  - Olesen NJ
FAU - Skall, Helle Frank
AU  - Skall HF
FAU - Jencic, Vlasta
AU  - Jencic V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
SB  - IM
MH  - Animals
MH  - Disease Outbreaks/*veterinary
MH  - Genotype
MH  - Hemorrhagic Septicemia, Viral/epidemiology/*virology
MH  - Novirhabdovirus/*genetics
MH  - *Oncorhynchus mykiss
MH  - Phylogeny
MH  - Slovenia/epidemiology
EDAT- 2010/12/21 06:00
MHDA- 2011/01/21 06:00
CRDT- 2010/12/21 06:00
PHST- 2010/12/21 06:00 [entrez]
PHST- 2010/12/21 06:00 [pubmed]
PHST- 2011/01/21 06:00 [medline]
AID - 10.3354/dao02251 [doi]
PST - ppublish
SO  - Dis Aquat Organ. 2010 Oct 26;92(1):21-9. doi: 10.3354/dao02251.