PMID- 21166694
OWN - NLM
STAT- MEDLINE
DCOM- 20110408
LR  - 20220317
IS  - 1365-2559 (Electronic)
IS  - 0309-0167 (Linking)
VI  - 57
IP  - 6
DP  - 2010 Dec
TI  - Association of mast cell, eosinophil leucocyte and microvessel densities in 
      actinic cheilitis and lip squamous cell carcinoma.
PG  - 796-805
LID - 10.1111/j.1365-2559.2010.03721.x [doi]
AB  - AIMS: To determine the contributions of mast cells (MC), eosinophil leucocytes 
      (EL) and microvessel density (MVD) in lip carcinogenesis, and to establish the 
      relationships between these biomarkers and their possible prognostic value in lip 
      squamous cell carcinoma (LSCC). METHODS AND RESULTS: Archived specimens of lip 
      mucosa (n=13), actinic cheilitis (n=29) and LSCC (n=29) were formalin-fixed, 
      paraffin-embedded, sectioned and stained with toluidine blue and haematoxylin and 
      eosin (H&E) in order to identify MC and EL and to measure their densities. Tumour 
      angiogenesis was estimated by determining, with the use of CD31 antibody MVD in 
      areas with the highest number of stained microvessels ('hot spots'). Progressive 
      increases of MC, EL and MVD were observed during lip tumour development. 
      Correlation analysis revealed positive associations between the biomarkers during 
      tumour progression. In LSCC samples, significant associations were found between 
      MVD values and metastatic disease. On multivariate analysis, MVD was a predictor 
      of risk of cervical metastasis. CONCLUSIONS: The densities of MC, EL and 
      microvessels increase during lip carcinogenesis, and for MC and EL this may be 
      related to the stimulation of tumour angiogenesis. MVD could be a useful 
      predictor of cervical metastasis in LSCC.
CI  - (c) 2010 Blackwell Publishing Limited.
FAU - Souza, Ludmilla R
AU  - Souza LR
AD  - Health Science Programme, State University of Montes Claros, Montes Claros, MG, 
      Brazil.
FAU - Fonseca-Silva, Thiago
AU  - Fonseca-Silva T
FAU - Santos, Carolina C O
AU  - Santos CC
FAU - Oliveira, Marcos V M
AU  - Oliveira MV
FAU - Correa-Oliveira, Rodrigo
AU  - Correa-Oliveira R
FAU - Guimaraes, Andre L S
AU  - Guimaraes AL
FAU - De Paula, Alfredo M B
AU  - De Paula AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Histopathology
JT  - Histopathology
JID - 7704136
RN  - Actinic cheilitis
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*pathology
MH  - Cheilitis/pathology
MH  - Eosinophils/*pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Lip Neoplasms/*pathology
MH  - Male
MH  - Mast Cells/*pathology
MH  - Microvessels
MH  - Middle Aged
MH  - Mouth Mucosa/pathology
MH  - Neovascularization, Pathologic/*pathology
EDAT- 2010/12/21 06:00
MHDA- 2011/04/09 06:00
CRDT- 2010/12/21 06:00
PHST- 2010/12/21 06:00 [entrez]
PHST- 2010/12/21 06:00 [pubmed]
PHST- 2011/04/09 06:00 [medline]
AID - 10.1111/j.1365-2559.2010.03721.x [doi]
PST - ppublish
SO  - Histopathology. 2010 Dec;57(6):796-805. doi: 10.1111/j.1365-2559.2010.03721.x.