PMID- 21166721
OWN - NLM
STAT- MEDLINE
DCOM- 20110621
LR  - 20211020
IS  - 1600-0625 (Electronic)
IS  - 0906-6705 (Linking)
VI  - 20
IP  - 2
DP  - 2011 Feb
TI  - The role of mitogen- and stress-activated protein kinase 1 and 2 in chronic skin 
      inflammation in mice.
PG  - 140-5
LID - 10.1111/j.1600-0625.2010.01153.x [doi]
AB  - Mitogen- and stress-activated protein kinase 1 and 2 (MSK1/2) are two kinases 
      phosphorylated by both ERK1/2 and p38 MAPK. Recently, MSK1 and 2 have been 
      reported to act as negative regulators of acute inflammation. In this study, we 
      investigated the role of MSK1/2 in chronic skin inflammation using an 
      oxazolone-induced allergic contact dermatitis model in MSK1/2 knockout mice and 
      wild-type mice. MSK1/2 knockout mice were demonstrated to have significantly 
      increased inflammation compared with wild-type mice. This was measured by an 
      increased ear thickness, elevated infiltration of neutrophils in the skin and 
      increased inflammatory histological changes. Furthermore, we found significantly 
      elevated levels of the proinflammatory cytokines Tumor necrosis factor-alpha (TNF-alpha), 
      IL-1beta and IL-6 at both mRNA and protein levels in MSK1/2 knockout mice compared 
      with wild-type mice after oxazolone treatment. In addition, the mRNA expression 
      of the chemokine Thymus and activation regulated chemokine (TARC) was 
      demonstrated to be significantly elevated in oxazolone-treated MSK1/2 knockout 
      mice compared with wild-type mice. The increased expression of TARC was 
      paralleled by increased infiltration of cells positive for the TARC receptor, 
      CCR4, in the dermis of MSK1/2 knockout mice. Our results indicate that MSK1/2 are 
      involved in the activation of feedback mechanisms that dampen oxazolone-induced 
      skin inflammation.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Bertelsen, Trine
AU  - Bertelsen T
AD  - Department of Dermatology, Aarhus University Hospital, Aarhus, Denmark.
FAU - Iversen, Lars
AU  - Iversen L
FAU - Riis, Jette Lindorff
AU  - Riis JL
FAU - Arthur, J Simon C
AU  - Arthur JS
FAU - Bibby, Bo Martin
AU  - Bibby BM
FAU - Kragballe, Knud
AU  - Kragballe K
FAU - Johansen, Claus
AU  - Johansen C
LA  - eng
GR  - MC_U127081014/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101217
PL  - Denmark
TA  - Exp Dermatol
JT  - Experimental dermatology
JID - 9301549
RN  - 0 (Ccl17 protein, mouse)
RN  - 0 (Ccl27a protein, mouse)
RN  - 0 (Chemokine CCL17)
RN  - 0 (Chemokine CCL27)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 15646-46-5 (Oxazolone)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 90-kDa)
RN  - EC 2.7.11.1 (Rps6ka4 protein, mouse)
RN  - EC 2.7.11.1 (mitogen and stress-activated protein kinase 1)
SB  - IM
MH  - Animals
MH  - Chemokine CCL17/metabolism
MH  - Chemokine CCL27/metabolism
MH  - Dermatitis, Allergic Contact/metabolism/pathology/*physiopathology
MH  - Disease Models, Animal
MH  - Female
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Oxazolone/*adverse effects
MH  - Ribosomal Protein S6 Kinases, 90-kDa/genetics/*physiology
MH  - Skin/metabolism/pathology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2010/12/21 06:00
MHDA- 2011/06/22 06:00
CRDT- 2010/12/21 06:00
PHST- 2010/12/21 06:00 [entrez]
PHST- 2010/12/21 06:00 [pubmed]
PHST- 2011/06/22 06:00 [medline]
AID - 10.1111/j.1600-0625.2010.01153.x [doi]
PST - ppublish
SO  - Exp Dermatol. 2011 Feb;20(2):140-5. doi: 10.1111/j.1600-0625.2010.01153.x. Epub 
      2010 Dec 17.