PMID- 21166802
OWN - NLM
STAT- MEDLINE
DCOM- 20110503
LR  - 20101220
IS  - 1601-5223 (Electronic)
IS  - 0018-0661 (Linking)
VI  - 147
IP  - 6
DP  - 2010 Dec
TI  - PRINS detection of 18S rDNA in pig, red fox and Chinese raccoon dog, and 
      centromere DNA in horse.
PG  - 320-4
LID - 10.1111/j.1601-5223.2010.02201.x [doi]
AB  - The fluorescence in situ hybridization (FISH) technique is widely used in animal 
      cytogenetics. Contrary to FISH procedure, primed in situ DNA synthesis (PRINS) 
      does not require the DNA probe preparation (design, synthesis, gel purification 
      of PCR products and labeling). The PRINS method with primers used as 'DNA probes' 
      is both PCR-sensitive and allows for chromosomal localization of DNA sequences. 
      Here, we show the application of PRINS reaction with one unlabeled 
      oligonucleotide pair to identify 18S rDNA loci in three different animal species: 
      domestic pig (Sus scrofa), red fox (Vulpes vulpes) and Chinese raccoon dog 
      (Nyctereutes procyonoides procyonoides). We present the data of indirect labeling 
      with the digoxigenin-PRINS using two different pairs of primers complementary to 
      centromeric region of horse (Equus caballus) chromosomes. Our new PRINS 
      application may be considered as a useful tool for chromosome investigation in 
      the field of domestic and wild animal genetics and evolution.
CI  - (c) 2010 The Authors.
FAU - Wnuk, Maciej
AU  - Wnuk M
AD  - Department of Genetics, University of Rzeszow, Rzeszow, Poland.
FAU - Oklejewicz, Bernadetta
AU  - Oklejewicz B
FAU - Lewinska, Anna
AU  - Lewinska A
FAU - Zabek, Tomasz
AU  - Zabek T
FAU - Bartosz, Grzegorz
AU  - Bartosz G
FAU - Slota, Ewa
AU  - Slota E
FAU - Bugno-Poniewierska, Monika
AU  - Bugno-Poniewierska M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101215
PL  - England
TA  - Hereditas
JT  - Hereditas
JID - 0374654
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 18S)
SB  - IM
MH  - Animals
MH  - Centromere/*genetics
MH  - Chromosomes, Mammalian/*genetics
MH  - DNA, Ribosomal/*genetics
MH  - Foxes
MH  - Horses
MH  - Primed In Situ Labeling
MH  - RNA, Ribosomal, 18S/*genetics
MH  - Raccoon Dogs
MH  - Species Specificity
MH  - Swine
EDAT- 2010/12/21 06:00
MHDA- 2011/05/04 06:00
CRDT- 2010/12/21 06:00
PHST- 2010/12/21 06:00 [entrez]
PHST- 2010/12/21 06:00 [pubmed]
PHST- 2011/05/04 06:00 [medline]
AID - 10.1111/j.1601-5223.2010.02201.x [doi]
PST - ppublish
SO  - Hereditas. 2010 Dec;147(6):320-4. doi: 10.1111/j.1601-5223.2010.02201.x. Epub 
      2010 Dec 15.