PMID- 21167474
OWN - NLM
STAT- MEDLINE
DCOM- 20110810
LR  - 20211025
IS  - 1879-1506 (Electronic)
IS  - 0003-9969 (Linking)
VI  - 56
IP  - 4
DP  - 2011 Apr
TI  - Fluoride reduces the expression of enamel proteins and cytokines in an 
      ameloblast-derived cell line.
PG  - 324-30
LID - 10.1016/j.archoralbio.2010.10.024 [doi]
AB  - OBJECTIVE: To investigate the effects of two different fluoride concentrations on 
      the expression of enamel proteins, alkaline phosphatase (ALP), cytokines and 
      interleukins by an ameloblast-derived cell line. METHODS: Murine 
      ameloblast-derived cells (LS-8), mouse odontogenic epithelia, were exposed to 1 
      or 5ppm sodium fluoride (NaF) (0.46 and 2.25ppm F, respectively) for 1, 3 and 7 
      days. The effect of NaF on the mRNA expression of enamel proteins was quantified; 
      the secretion of cytokines, and interleukins, and the alkaline phosphatase (ALP) 
      activity, into the cell culture medium was measured and compared to untreated 
      controls. The effect on cell growth after 1- and 3-days in culture was measured 
      using BrdU incorporation. RESULTS: Fluoride at 2.25ppm reduced mRNA expression of 
      the structural enamel matrix proteins amelogenin (amel), ameloblastin (ambn), 
      enamelin (enam), and the enamel protease matrix metallopeptidase-20 (MMP-20). 
      Similarly several vascularisation factors (vascular endothelial growth factor 
      (VEGF), monocyte chemoattractant proteins (MCP-1) and interferon inducible 
      protein 10 (IP-10), was also reduced by 2.25ppm fluoride. ALP activity and 
      proliferation were stimulated by 0.46ppm fluoride but inhibited by 2.25ppm 
      fluoride. CONCLUSIONS: These results indicate that fluoride may impact on the 
      expression of structural enamel proteins and the protease responsible for 
      processing these proteins during the secretory stage of amelogenesis and go some 
      way to explaining the mineralization defect that characterises fluorotic enamel.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Riksen, Elisabeth Aurstad
AU  - Riksen EA
AD  - Department of Biomaterials, Faculty of Dentistry, University of Oslo, Norway.
FAU - Kalvik, Anne
AU  - Kalvik A
FAU - Brookes, Steven
AU  - Brookes S
FAU - Hynne, Astrid
AU  - Hynne A
FAU - Snead, Malcolm L
AU  - Snead ML
FAU - Lyngstadaas, S Petter
AU  - Lyngstadaas SP
FAU - Reseland, Janne E
AU  - Reseland JE
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101217
PL  - England
TA  - Arch Oral Biol
JT  - Archives of oral biology
JID - 0116711
RN  - 0 (Cariostatic Agents)
RN  - 0 (Cytokines)
RN  - 0 (Dental Enamel Proteins)
RN  - 0 (Interleukins)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alkaline Phosphatase/drug effects/metabolism
MH  - Ameloblasts/cytology/*drug effects/metabolism
MH  - Animals
MH  - Calcium/metabolism
MH  - Cariostatic Agents/*pharmacology
MH  - Cell Line
MH  - Cell Proliferation/*drug effects
MH  - Cytokines/drug effects/metabolism
MH  - Dental Enamel Proteins/*drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Interleukins/metabolism
MH  - L-Lactate Dehydrogenase/drug effects/metabolism
MH  - Mice
MH  - Sodium Fluoride/*pharmacology
EDAT- 2010/12/21 06:00
MHDA- 2011/08/11 06:00
CRDT- 2010/12/21 06:00
PHST- 2010/05/14 00:00 [received]
PHST- 2010/10/19 00:00 [revised]
PHST- 2010/10/31 00:00 [accepted]
PHST- 2010/12/21 06:00 [entrez]
PHST- 2010/12/21 06:00 [pubmed]
PHST- 2011/08/11 06:00 [medline]
AID - S0003-9969(10)00335-3 [pii]
AID - 10.1016/j.archoralbio.2010.10.024 [doi]
PST - ppublish
SO  - Arch Oral Biol. 2011 Apr;56(4):324-30. doi: 10.1016/j.archoralbio.2010.10.024. 
      Epub 2010 Dec 17.