PMID- 21167926
OWN - NLM
STAT- MEDLINE
DCOM- 20111107
LR  - 20220311
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 134
IP  - 2
DP  - 2011 Mar 24
TI  - Panax notoginseng saponins promotes proliferation and osteogenic differentiation 
      of rat bone marrow stromal cells.
PG  - 268-74
LID - 10.1016/j.jep.2010.11.075 [doi]
AB  - AIM OF THE STUDY: Panax notoginseng saponins (PNS) is the main effective 
      component of Panax notoginseng, have various pharmacologic activities such as 
      antioxidant, anti-inflammatory, and estrogen-like bioactivities, have been shown 
      to be an effective agent on anti-osteoporosis. Bone marrow stromal cells (BMSCs) 
      play a crucial homeostatic role in skeletal modeling and remodeling due to their 
      capability to differentiate into osteooblasts. Whether PNS has effect on 
      osteogenic differentiation of BMSCs are unknown. This study was designed to 
      investigate the effects of PNS on the proliferation and osteogenic 
      differentiation of BMSCs in vitro. MATERIALS AND METHODS: When BMSCs cultivated 
      in the basal medium or the osteogenic induction medium (OS with or without PNS), 
      cell proliferation was analyzed using an MTT assay, the mineralization was 
      assessed using Alizarin red S staining, the alkaline phosphatase activity was 
      measured using a commercial kit, the mRNA level of osteogenic gene and PPARgamma2 
      gene were determined using RT-PCR, the protein level of PPARgamma2 was analyzed by 
      Western blotting. RESULTS: BMSCs cultured in the basal medium with PNS caused a 
      significant increase in proliferation. PNS treatment increased ALP activity, 
      Alizarin red S staining and mRNA level of ALP, Cbfa 1, OC, and BSP, whereas 
      decreased the mRNA level and protein expression of PPARgamma2 during osteogenic 
      induction. In addition, the effects of PNS treatment were dose-dependent 
      relationship. CONCLUSION: PNS could stimulate BMSCs proliferation and promote 
      their osteogenic differentiation by up-regulation expression of osteogenic marker 
      gene and down-regulation expression of adipogenic marker gene in a dose-dependent 
      manner. Thus, PNS may play an important therapeutic role in osteoporosis patients 
      by improving osteogenic differentiation of BMSCs.
CI  - Crown Copyright (c) 2010. Published by Elsevier Ireland Ltd. All rights reserved.
FAU - Li, Xue-Dong
AU  - Li XD
AD  - Department of Orthopaedics, The First Affiliated Hospital, Shantou University 
      Medical College, 57 Chang Ping Road, Shantou, Guangdong 515041, China.
FAU - Wang, Jian-Sheng
AU  - Wang JS
FAU - Chang, Bo
AU  - Chang B
FAU - Chen, Bin
AU  - Chen B
FAU - Guo, Chun
AU  - Guo C
FAU - Hou, Guo-Qing
AU  - Hou GQ
FAU - Huang, Dong-Yang
AU  - Huang DY
FAU - Du, Shi-Xin
AU  - Du SX
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101216
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Bone Density Conservation Agents)
RN  - 0 (Core Binding Factor alpha Subunits)
RN  - 0 (Integrin-Binding Sialoprotein)
RN  - 0 (PPAR gamma)
RN  - 0 (Plant Extracts)
RN  - 0 (RNA, Messenger)
RN  - 0 (Saponins)
RN  - 0 (core binding factor alpha)
RN  - 104982-03-8 (Osteocalcin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/genetics/metabolism
MH  - Animals
MH  - Bone Density Conservation Agents/pharmacology/therapeutic use
MH  - Bone Marrow Cells/*drug effects/metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Core Binding Factor alpha Subunits/genetics/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Integrin-Binding Sialoprotein/genetics/metabolism
MH  - Mesenchymal Stem Cells/*drug effects/metabolism
MH  - Osteocalcin/genetics/metabolism
MH  - Osteogenesis/*drug effects
MH  - Osteoporosis/*drug therapy/metabolism
MH  - PPAR gamma/genetics/metabolism
MH  - Panax notoginseng/*chemistry
MH  - Phytotherapy
MH  - Plant Extracts/*pharmacology/therapeutic use
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Saponins/*pharmacology/therapeutic use
MH  - Stromal Cells/drug effects/metabolism
EDAT- 2010/12/21 06:00
MHDA- 2011/11/08 06:00
CRDT- 2010/12/21 06:00
PHST- 2010/06/10 00:00 [received]
PHST- 2010/11/04 00:00 [revised]
PHST- 2010/11/21 00:00 [accepted]
PHST- 2010/12/21 06:00 [entrez]
PHST- 2010/12/21 06:00 [pubmed]
PHST- 2011/11/08 06:00 [medline]
AID - S0378-8741(10)00873-1 [pii]
AID - 10.1016/j.jep.2010.11.075 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2011 Mar 24;134(2):268-74. doi: 10.1016/j.jep.2010.11.075. Epub 
      2010 Dec 16.