PMID- 21169227
OWN - NLM
STAT- MEDLINE
DCOM- 20110223
LR  - 20230216
IS  - 1541-6100 (Electronic)
IS  - 0022-3166 (Linking)
VI  - 141
IP  - 2
DP  - 2011 Feb
TI  - Dietary manganese modulates expression of the manganese-containing superoxide 
      dismutase gene in chickens.
PG  - 189-94
LID - 10.3945/jn.110.126680 [doi]
AB  - To investigate the possible mechanism(s) by which dietary manganese (Mn) levels 
      and sources modulate the expression of the manganese-containing superoxide 
      dismutase (MnSOD) gene at both the transcriptional and translational levels, we 
      used 432 8-d-old male broiler chicks in a 1 plus 4 x 2 design. Chickens were 
      given either a diet without Mn supplementation [control (C)] or diets 
      supplemented with 100 (optimal) or 200 (high) mg Mn/kg diet from inorganic Mn 
      sulfate (I) or 3 organic complexes of Mn and amino acids with weak (W), moderate 
      (M), or strong (S) chelation strength up to 21 d of age. Compared with C chicks, 
      chicks fed Mn-supplemented diets had higher (P < 0.01) Mn concentrations, 
      specificity protein 1 (Sp1) DNA-binding activities, MnSOD mRNA levels, MnSOD 
      mRNA-binding protein (MnSOD-BP) RNA-binding activities, MnSOD protein 
      concentrations, and MnSOD activities within heart tissue, but lower (P < 0.01) 
      heart activating protein-2 (AP-2) DNA-binding activities. Chicks fed M diets had 
      higher (P < 0.05) heart Mn concentrations, MnSOD mRNA levels, and MnSOD-BP 
      RNA-binding activities compared with those fed the I and W diets and lower (P < 
      0.01) AP-2 DNA-binding activities than those fed other treatment diets. These 
      results suggest that dietary Mn could modulate the expression of the MnSOD gene 
      in broilers by altering Sp1 and AP-2 DNA-binding activities at the 
      transcriptional level and enhancing MnSOD-BP RNA-binding activity at the 
      translational level. Additionally, an organic Mn source with moderate chelation 
      strength could be more effective than other Mn sources in activating MnSOD gene 
      expression at both the transcriptional and translational levels.
FAU - Li, Sufen
AU  - Li S
AD  - Mineral Nutrition Research Division, State Key Laboratory of Animal Nutrition, 
      Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, P 
      R China.
FAU - Lu, Lin
AU  - Lu L
FAU - Hao, Shoufeng
AU  - Hao S
FAU - Wang, Yanping
AU  - Wang Y
FAU - Zhang, Liyang
AU  - Zhang L
FAU - Liu, Songbai
AU  - Liu S
FAU - Liu, Bin
AU  - Liu B
FAU - Li, Kui
AU  - Li K
FAU - Luo, Xugang
AU  - Luo X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101215
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Amino Acids)
RN  - 0 (Chelating Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (Transcription Factor AP-2)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 7487-88-9 (Magnesium Sulfate)
RN  - 9007-49-2 (DNA)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Amino Acids/pharmacology
MH  - Animals
MH  - Chelating Agents/*pharmacology
MH  - Chickens
MH  - DNA/metabolism
MH  - *Diet
MH  - Dietary Supplements
MH  - Gene Expression/*drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Magnesium Sulfate/pharmacology
MH  - Manganese/metabolism/*pharmacology
MH  - Myocardium/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Sp1 Transcription Factor/genetics/metabolism
MH  - Superoxide Dismutase/genetics/*metabolism
MH  - Transcription Factor AP-2/genetics/metabolism
EDAT- 2010/12/21 06:00
MHDA- 2011/02/24 06:00
CRDT- 2010/12/21 06:00
PHST- 2010/12/21 06:00 [entrez]
PHST- 2010/12/21 06:00 [pubmed]
PHST- 2011/02/24 06:00 [medline]
AID - S0022-3166(22)02471-3 [pii]
AID - 10.3945/jn.110.126680 [doi]
PST - ppublish
SO  - J Nutr. 2011 Feb;141(2):189-94. doi: 10.3945/jn.110.126680. Epub 2010 Dec 15.