PMID- 21175635
OWN - NLM
STAT- MEDLINE
DCOM- 20141231
LR  - 20231213
IS  - 1469-8137 (Electronic)
IS  - 0028-646X (Linking)
VI  - 190
IP  - 1
DP  - 2011 Apr
TI  - An elaborate heterotrimeric G-protein family from soybean expands the diversity 
      of plant G-protein networks.
PG  - 35-48
LID - 10.1111/j.1469-8137.2010.03581.x [doi]
AB  - The repertoire of heterotrimeric G-proteins in plant species analyzed thus far is 
      simple, with the presence of only two possible canonical heterotrimers in 
      Arabidopsis and rice vs hundreds in animal systems. We assessed whether genome 
      duplication events have resulted in the multiplicity of G-protein in plant 
      species like soybean that would increase the complexity of G-protein networks. We 
      identified and amplified four Galpha, four Gbeta and two Ggamma proteins, analyzed their 
      expression profile by quantitative PCR during different developmental stages. We 
      purified the four Galpha proteins and analyzed their guanosine-5'-triphosphate 
      (GTP)-binding and GTPase activity. We performed yeast-based interaction analysis 
      to assess the interaction specificity of different G-protein subunits. Our 
      results show that all 10 G-protein genes are retained in the soybean genome and 
      ubiquitously expressed. The four Galpha proteins seem to be plasma 
      membrane-localized. The G-protein genes have interesting expression profiles 
      during seed development and germination. The four Galpha proteins form two distinct 
      groups based on their GTPase activity. Yeast-based interaction analyses predict 
      that the proteins interact in most of the possible combinations, with some degree 
      of interaction specificity between duplicated gene pairs. This research 
      identifies the most elaborate heterotrimeric G-protein network known to date in 
      the plant kingdom.
CI  - (c) 2010 The Authors. New Phytologist (c) 2010 New Phytologist Trust.
FAU - Bisht, Naveen C
AU  - Bisht NC
AD  - Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, 
      USA.
FAU - Jez, Joseph M
AU  - Jez JM
AD  - Department of Biology, Washington University, One Brookings Drive, Campus Box 
      1137, St Louis, MO 63130, USA.
FAU - Pandey, Sona
AU  - Pandey S
AD  - Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO 63132, 
      USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20101222
PL  - England
TA  - New Phytol
JT  - The New phytologist
JID - 9882884
RN  - 0 (Plant Proteins)
RN  - 0 (Protein Subunits)
RN  - 0 (Recombinant Proteins)
RN  - 72S9A8J5GW (Abscisic Acid)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.6.5.1 (Heterotrimeric GTP-Binding Proteins)
SB  - IM
CIN - New Phytol. 2011 Apr;190(1):1-3. PMID: 24079893
MH  - Abscisic Acid/pharmacology
MH  - Amino Acid Sequence
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Plant/drug effects
MH  - Genes, Plant
MH  - Germination/drug effects/genetics
MH  - Guanosine Triphosphate/metabolism
MH  - Heterotrimeric GTP-Binding Proteins/genetics/isolation & purification/*metabolism
MH  - Hydrolysis/drug effects
MH  - Molecular Sequence Data
MH  - *Multigene Family
MH  - Plant Proteins/genetics/*metabolism
MH  - Protein Interaction Mapping
MH  - Protein Subunits/genetics/*metabolism
MH  - Protein Transport/drug effects
MH  - Recombinant Proteins/isolation & purification/metabolism
MH  - Seeds/drug effects/genetics/growth & development
MH  - Sequence Alignment
MH  - *Signal Transduction/drug effects/genetics
MH  - Glycine max/drug effects/genetics/*metabolism
MH  - Stress, Physiological/drug effects/genetics
OTO - NOTNLM
OT  - GTP-binding
OT  - GTPase activity
OT  - genome duplication
OT  - heterotrimeric G-proteins
OT  - protein-protein interaction
OT  - soybean (Glycine max)
EDAT- 2010/12/24 06:00
MHDA- 2015/01/01 06:00
CRDT- 2010/12/24 06:00
PHST- 2010/12/24 06:00 [entrez]
PHST- 2010/12/24 06:00 [pubmed]
PHST- 2015/01/01 06:00 [medline]
AID - 10.1111/j.1469-8137.2010.03581.x [doi]
PST - ppublish
SO  - New Phytol. 2011 Apr;190(1):35-48. doi: 10.1111/j.1469-8137.2010.03581.x. Epub 
      2010 Dec 22.