PMID- 21175696
OWN - NLM
STAT- MEDLINE
DCOM- 20110225
LR  - 20181201
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 75
IP  - 3
DP  - 2011 Mar
TI  - Bacterial diversity of field-caught mosquitoes, Aedes albopictus and Aedes 
      aegypti, from different geographic regions of Madagascar.
PG  - 377-89
LID - 10.1111/j.1574-6941.2010.01012.x [doi]
AB  - Symbiotic bacteria are known to play important roles in the biology of insects, 
      but the current knowledge of bacterial communities associated with mosquitoes is 
      very limited and consequently their contribution to host behaviors is mostly 
      unknown. In this study, we explored the composition and diversity of 
      mosquito-associated bacteria in relation with mosquitoes' habitats. Wild Aedes 
      albopictus and Aedes aegypti were collected in three different geographic regions 
      of Madagascar. Culturing methods and denaturing gradient gel electrophoresis 
      (DGGE) and sequencing of the rrs amplicons revealed that Proteobacteria and 
      Firmicutes were the major phyla. Isolated bacterial genera were dominated by 
      Bacillus, followed by Acinetobacter, Agrobacterium and Enterobacter. Common DGGE 
      bands belonged to Acinetobacter, Asaia, Delftia, Pseudomonas, Enterobacteriaceae 
      and an uncultured Gammaproteobacterium. Double infection by maternally inherited 
      Wolbachia pipientis prevailed in 98% of males (n=272) and 99% of females (n=413); 
      few individuals were found to be monoinfected with Wolbachia wAlbB strain. 
      Bacterial diversity (Shannon-Weaver and Simpson indices) differed significantly 
      per habitat whereas evenness (Pielou index) was similar. Overall, the bacterial 
      composition and diversity were influenced both by the sex of individuals and by 
      the environment inhabited by the mosquitoes; the latter might be related to both 
      the vegetation and the animal host populations that Aedes used as food sources.
CI  - (c) 2010 Federation of European Microbiological Societies. Published by Blackwell 
      Publishing Ltd. All rights reserved.
FAU - Zouache, Karima
AU  - Zouache K
AD  - Universite de Lyon, Lyon, France.
FAU - Raharimalala, Fara Nantenaina
AU  - Raharimalala FN
FAU - Raquin, Vincent
AU  - Raquin V
FAU - Tran-Van, Van
AU  - Tran-Van V
FAU - Raveloson, Lala Harivelo Ravaomanarivo
AU  - Raveloson LH
FAU - Ravelonandro, Pierre
AU  - Ravelonandro P
FAU - Mavingui, Patrick
AU  - Mavingui P
LA  - eng
SI  - GENBANK/GU726171
SI  - GENBANK/GU726172
SI  - GENBANK/GU726173
SI  - GENBANK/GU726174
SI  - GENBANK/GU726175
SI  - GENBANK/GU726176
SI  - GENBANK/GU726177
SI  - GENBANK/GU726178
SI  - GENBANK/GU726179
SI  - GENBANK/GU726180
SI  - GENBANK/GU726181
SI  - GENBANK/GU726182
SI  - GENBANK/GU726183
SI  - GENBANK/GU726184
SI  - GENBANK/GU726185
SI  - GENBANK/GU985109
SI  - GENBANK/GU985110
SI  - GENBANK/GU985111
SI  - GENBANK/GU985112
SI  - GENBANK/GU985113
SI  - GENBANK/GU985114
SI  - GENBANK/GU985115
SI  - GENBANK/GU985116
SI  - GENBANK/GU985117
SI  - GENBANK/GU985118
SI  - GENBANK/GU985119
SI  - GENBANK/GU985120
SI  - GENBANK/GU985121
SI  - GENBANK/GU985122
SI  - GENBANK/GU985123
SI  - GENBANK/GU985124
SI  - GENBANK/GU985125
SI  - GENBANK/GU985126
SI  - GENBANK/GU985127
SI  - GENBANK/GU985128
SI  - GENBANK/GU985129
SI  - GENBANK/GU985130
SI  - GENBANK/GU985131
SI  - GENBANK/GU985132
SI  - GENBANK/GU985133
SI  - GENBANK/GU985134
SI  - GENBANK/GU985135
SI  - GENBANK/GU985136
SI  - GENBANK/GU985137
SI  - GENBANK/GU985138
SI  - GENBANK/GU985139
SI  - GENBANK/GU985140
SI  - GENBANK/GU985141
SI  - GENBANK/GU985142
SI  - GENBANK/GU985143
SI  - GENBANK/GU985144
SI  - GENBANK/GU985145
SI  - GENBANK/GU985146
SI  - GENBANK/GU985147
SI  - GENBANK/GU985148
SI  - GENBANK/GU985149
SI  - GENBANK/GU985150
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101222
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Aedes/*microbiology
MH  - Animals
MH  - Bacteria/*classification/genetics/growth & development/isolation & purification
MH  - *Biodiversity
MH  - Denaturing Gradient Gel Electrophoresis
MH  - *Ecosystem
MH  - Female
MH  - Madagascar
MH  - Male
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Principal Component Analysis
MH  - RNA, Ribosomal, 16S/genetics
MH  - Wolbachia/classification/genetics/physiology
EDAT- 2010/12/24 06:00
MHDA- 2011/02/26 06:00
CRDT- 2010/12/24 06:00
PHST- 2010/12/24 06:00 [entrez]
PHST- 2010/12/24 06:00 [pubmed]
PHST- 2011/02/26 06:00 [medline]
AID - 10.1111/j.1574-6941.2010.01012.x [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2011 Mar;75(3):377-89. doi: 
      10.1111/j.1574-6941.2010.01012.x. Epub 2010 Dec 22.