PMID- 21176395
OWN - NLM
STAT- MEDLINE
DCOM- 20120308
LR  - 20111122
IS  - 1555-3892 (Electronic)
IS  - 0963-6897 (Linking)
VI  - 20
IP  - 9
DP  - 2011
TI  - Replacement of liver parenchyma in analbuminemic rats with allogenic hepatocytes 
      is facilitated by intrabone marrow-bone marrow transplantation.
PG  - 1479-89
LID - 10.3727/096368910X547453 [doi]
AB  - Although hepatocyte transplantation (HCTx) is expected to become a useful therapy 
      for human liver diseases, allogenic hepatocytes still tend to be rejected within 
      a short period due to host immunosurveillance. In the present study, we 
      investigated the effect of prior bone marrow transplantation (BMTx) for the 
      engraftment of allogenic hepatocytes using the analbuminemic rat transplantation 
      model. The hepatocytes of Lewis (LEW) rats were not accepted in the liver of 
      retrorsine (RS)/partial hepatectomy (PH)-treated analbuminemic F344 (F344-alb) 
      rats, which express the disparate major histocompatibility complex (MHC) against 
      that of LEW rats. Prior BMTx with the LEW bone marrow cells (BMCs) after 
      sublethal irradiation achieved acceptance and repopulation of LEW hepatocytes in 
      the liver of the RS/PH-treated F344-alb rats, associated with elevation of serum 
      albumin. The replacement of hepatic parenchyma with albumin positive (Alb(+)) 
      donor hepatocytes and elevation of serum albumin levels were dependent on the 
      bone marrow reconstitution by donor BMCs, which was more efficiently achieved by 
      intrabone marrow (IBM)-BMTx than by intravenous (IV)-BMTx. Our results 
      demonstrate that efficient bone marrow reconstitution by IBM-BMTx enables the 
      replacement of the hepatic parenchyma with allogenic hepatocytes in RS/PH-treated 
      analbuminemic rats without immunosuppressants.
FAU - Inagaki, Mitsuhiro
AU  - Inagaki M
AD  - Department of Surgery, Asahikawa Medical University, Asahikawa, Japan. 
      inagaki@asahikawa-med.ac.jp
FAU - Furukawa, Hiroyuki
AU  - Furukawa H
FAU - Satake, Yoshiyasu
AU  - Satake Y
FAU - Okada, Yoko
AU  - Okada Y
FAU - Chiba, Shinichi
AU  - Chiba S
FAU - Nishikawa, Yuji
AU  - Nishikawa Y
FAU - Ogawa, Katsuhiro
AU  - Ogawa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101222
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Animals
MH  - Bone Marrow/*metabolism
MH  - *Bone Marrow Transplantation
MH  - Hepatocytes/metabolism/*transplantation
MH  - Humans
MH  - Liver/metabolism/*pathology
MH  - Male
MH  - Rats
MH  - Rats, Inbred F344
MH  - Rats, Inbred Lew
MH  - Serum Albumin/*metabolism
MH  - Survival Analysis
MH  - Transplantation, Homologous
EDAT- 2010/12/24 06:00
MHDA- 2012/03/09 06:00
CRDT- 2010/12/24 06:00
PHST- 2010/12/24 06:00 [entrez]
PHST- 2010/12/24 06:00 [pubmed]
PHST- 2012/03/09 06:00 [medline]
AID - ct0082inagaki [pii]
AID - 10.3727/096368910X547453 [doi]
PST - ppublish
SO  - Cell Transplant. 2011;20(9):1479-89. doi: 10.3727/096368910X547453. Epub 2010 Dec 
      22.