PMID- 21177252
OWN - NLM
STAT- MEDLINE
DCOM- 20110617
LR  - 20211020
IS  - 1096-0929 (Electronic)
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 120
IP  - 1
DP  - 2011 Mar
TI  - Manganese uptake and distribution in the brain after methyl bromide-induced 
      lesions in the olfactory epithelia.
PG  - 163-72
LID - 10.1093/toxsci/kfq387 [doi]
AB  - Manganese (Mn) is an essential nutrient with potential neurotoxic effects. Mn 
      deposited in the nose is apparently transported to the brain through anterograde 
      axonal transport, bypassing the blood-brain barrier. However, the role of the 
      olfactory epithelial cells in Mn transport from the nasal cavity to the blood and 
      brain is not well understood. We utilized the methyl bromide (MeBr) lesion model 
      wherein the olfactory epithelium fully regenerates in a time-dependent and cell 
      type-specific manner over the course of 6-8 weeks postinjury. We instilled 
      (54)MnCl(2) intranasally at different recovery periods to study the role of 
      specific olfactory epithelial cell types in Mn transport. (54)MnCl(2) was 
      instilled at 2, 4, 7, 21, and 56 days post-MeBr treatment. (54)Mn concentrations 
      in the blood were measured over the first 4-h period and in the brain and other 
      tissues at 7 days postinstillation. Age-matched control rats were similarly 
      studied at 2 and 56 days. Blood and tissue (54)Mn levels were reduced initially 
      but returned to control values by day 7 post-MeBr exposure, coinciding with the 
      reestablishment of sustentacular cells. Brain (54)Mn levels also decreased but 
      returned to control levels only by 21 days, the period near the completion of 
      neuronal regeneration/bulbar reinnervation. Our data show that Mn transport to 
      the blood and brain temporally correlated with olfactory epithelial regeneration 
      post-MeBr injury. We conclude that (1) sustentacular cells are necessary for Mn 
      transport to the blood and (2) intact axonal projections are required for Mn 
      transport from the nasal cavity to the olfactory bulb and brain.
FAU - Thompson, Khristy J
AU  - Thompson KJ
AD  - Molecular and Integrative Physiological Sciences Program, Department of 
      Environmental Health, Harvard School of Public Health, Boston, Massachusetts 
      02115, USA.
FAU - Molina, Ramon M
AU  - Molina RM
FAU - Donaghey, Thomas
AU  - Donaghey T
FAU - Savaliya, Sandeep
AU  - Savaliya S
FAU - Schwob, James E
AU  - Schwob JE
FAU - Brain, Joseph D
AU  - Brain JD
LA  - eng
GR  - R01 DC002167/DC/NIDCD NIH HHS/United States
GR  - ES000002/ES/NIEHS NIH HHS/United States
GR  - P01 ES012874/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20101220
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Chlorides)
RN  - 0 (Hydrocarbons, Brominated)
RN  - 0 (Manganese Compounds)
RN  - 9V42E1Z7B6 (methyl bromide)
RN  - QQE170PANO (manganese chloride)
SB  - IM
MH  - Administration, Intranasal
MH  - Aging/blood/metabolism
MH  - Animals
MH  - Brain/*metabolism
MH  - Chlorides/administration & dosage/blood/*pharmacokinetics
MH  - *Hydrocarbons, Brominated/toxicity
MH  - Injections, Intravenous
MH  - Male
MH  - Manganese Compounds/administration & dosage/blood/*pharmacokinetics
MH  - Olfactory Mucosa/drug effects/*metabolism/pathology/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Regeneration/physiology
MH  - Tissue Distribution
PMC - PMC3044207
EDAT- 2010/12/24 06:00
MHDA- 2011/06/18 06:00
PMCR- 2012/03/01
CRDT- 2010/12/24 06:00
PHST- 2010/12/24 06:00 [entrez]
PHST- 2010/12/24 06:00 [pubmed]
PHST- 2011/06/18 06:00 [medline]
PHST- 2012/03/01 00:00 [pmc-release]
AID - kfq387 [pii]
AID - 10.1093/toxsci/kfq387 [doi]
PST - ppublish
SO  - Toxicol Sci. 2011 Mar;120(1):163-72. doi: 10.1093/toxsci/kfq387. Epub 2010 Dec 
      20.