PMID- 21177980
OWN - NLM
STAT- MEDLINE
DCOM- 20111108
LR  - 20220311
IS  - 1535-4989 (Electronic)
IS  - 1044-1549 (Print)
IS  - 1044-1549 (Linking)
VI  - 45
IP  - 3
DP  - 2011 Sep
TI  - Dendritic cell depletion and repopulation in the lung after irradiation and bone 
      marrow transplantation in mice.
PG  - 534-41
LID - 10.1165/rcmb.2010-0279OC [doi]
AB  - Dendritic cells (DCs) are essential for innate and adaptive immunity, but are 
      purported to exhibit variable radiosensitivity in response to irradiation in 
      various bone marrow transplantation (BMT) protocols. To address this controversy, 
      we analyzed the magnitude of depletion and repopulation of both lung CD11b(pos) 
      DC and CD103(pos) DC subsets in response to irradiation and BMT in a murine 
      model. In our study, CD45.2(pos) donor bone marrow cells were transplanted into 
      irradiated CD45.1(pos) recipient mice to examine the depletion of recipient DC 
      subsets and the repopulation of donor DC subsets. We observed an 
      apoptosis-mediated and necrosis-mediated depletion (> 90%) of the recipient 
      CD103(pos) DC subset, and only a 50-60% depletion of recipient CD11b(pos) DCs 
      from lung parenchymal tissue on Days 3 and 5, whereas recipient alveolar and lung 
      macrophages were much less radiosensitive, showing an approximately 50% depletion 
      by Days 14-21 after treatment. A repopulation of lung tissue with donor DC 
      subsets had occurred by Days 10 and 28 for CD11b(pos) DCs and CD103(pos) DCs, 
      whereas alveolar and lung macrophages were repopulated by 6 and 10 weeks after 
      treatment. Furthermore, the infection of mice with Streptococcus pneumoniae 
      further accelerated the turnover of lung DCs and lung macrophage subsets. Our 
      data illustrate the vulnerability of lung CD103(pos) DCs and CD11b(pos) DCs to 
      irradiation, and indicate that an accelerated turnover of lung DC subsets occurs, 
      relative to pulmonary and lung macrophages. Our findings may have important 
      implications in the development of adjuvant immune-stimulatory protocols that 
      could reduce the risk of opportunistic infections in patients undergoing BMT.
FAU - Hahn, Ines
AU  - Hahn I
AD  - Department of Experimental Pneumology, Hannover School of Medicine, Hannover 
      30625, Germany.
FAU - Klaus, Anna
AU  - Klaus A
FAU - Maus, Regina
AU  - Maus R
FAU - Christman, John W
AU  - Christman JW
FAU - Welte, Tobias
AU  - Welte T
FAU - Maus, Ulrich A
AU  - Maus UA
LA  - eng
GR  - R01 HL075557/HL/NHLBI NIH HHS/United States
GR  - R01 HL103643/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20101222
PL  - United States
TA  - Am J Respir Cell Mol Biol
JT  - American journal of respiratory cell and molecular biology
JID - 8917225
RN  - 0 (Antigens, CD)
RN  - 0 (CD11b Antigen)
RN  - 0 (Integrin alpha Chains)
RN  - 0 (alpha E integrins)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - Antigens, CD/biosynthesis
MH  - Apoptosis
MH  - Bone Marrow Transplantation/*methods
MH  - CD11b Antigen/biosynthesis
MH  - Dendritic Cells/*cytology
MH  - Immunophenotyping
MH  - Integrin alpha Chains/biosynthesis
MH  - Leukocyte Common Antigens/biosynthesis
MH  - Lung/*pathology
MH  - Macrophages/cytology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Models, Biological
MH  - Necrosis
MH  - Streptococcus pneumoniae/metabolism
MH  - Time Factors
PMC - PMC3361352
EDAT- 2010/12/24 06:00
MHDA- 2011/11/09 06:00
PMCR- 2012/09/01
CRDT- 2010/12/24 06:00
PHST- 2010/12/24 06:00 [entrez]
PHST- 2010/12/24 06:00 [pubmed]
PHST- 2011/11/09 06:00 [medline]
PHST- 2012/09/01 00:00 [pmc-release]
AID - 2010-0279OC [pii]
AID - 10.1165/rcmb.2010-0279OC [doi]
PST - ppublish
SO  - Am J Respir Cell Mol Biol. 2011 Sep;45(3):534-41. doi: 10.1165/rcmb.2010-0279OC. 
      Epub 2010 Dec 22.