PMID- 21181220
OWN - NLM
STAT- MEDLINE
DCOM- 20110922
LR  - 20211203
IS  - 1434-9949 (Electronic)
IS  - 0770-3198 (Linking)
VI  - 30
IP  - 6
DP  - 2011 Jun
TI  - Th-17 associated cytokines in patients with reactive arthritis/undifferentiated 
      spondyloarthropathy.
PG  - 771-6
LID - 10.1007/s10067-010-1646-5 [doi]
AB  - We and others have previously shown that IL-17 is elevated in the synovial fluid 
      of patients with reactive arthritis (ReA)/undifferentiated spondyloarthropathy 
      (uSpA) having acute synovitis. Major source for IL-17 is Th17 cells, which 
      differentiate from Th0 cells under the influence of TGF-beta and IL-6, IL1-beta and are 
      maintained by IL-21 and 23. There is a paucity of data on these cytokines in 
      ReA/uSpA. Thus, we measured the levels of Th-17 differentiating and maintaining 
      cytokines in synovial fluid of patients with ReA and uSpA. Fifty patients with 
      ReA/uSpA (ReA 24, uSpA 26), 19 patients with rheumatoid arthritis (RA) and 11 
      patients with osteoarthritis (OA) were included in the study. Synovial fluid (SF) 
      were collected from knee joint and stored at -80 degrees C until analysis. Cytokines were 
      assayed using ELISA in SF specimens. The median IL-17A levels were significantly 
      elevated in ReA (48.3 pg/ml) and uSpA (32.5 pg/ml) as compared to 
      non-inflammatory OA controls (<7.8 pg/ml; p < 0.0001), while comparable to RA 
      (57.9 pg/ml). Further, IL-6 median values were higher in ReA (25.2 ng/ml) and 
      uSpA (13.6 ng/ml) as compared to OA (0.76 ng/ml; p < 0.0001), and comparable to 
      RA (15.8 ng/ml). The median levels of IL-1beta, IL-21 levels were elevated in ReA, 
      uSpA and RA as compared to OA but were not statistically significant. TGF-beta 
      levels in ReA and uSpA were similar to OA but lower than in RA (4340 pg/ml; 
      p < 0.05). IL-23 was not detectable in any synovial fluid sample. However, levels 
      of these cytokines did not correlate with disease activity parameters. 
      Significant positive correlation was observed between IL-17 and IL-1beta (r = 0.38, 
      p < 0.005), IL-17 and IL-6 (r = 0.659, p < 0.0001), and IL-1beta and IL-6 
      (r = 0.391, p < 0.0001) in ReA and uSpA group. Inflammatory synovitis in ReA/uSpA 
      is mediated by pro-inflammatory cytokines like IL-17, IL-6, IL-1beta, and IL-21. 
      However, IL-23 was not detectable in SF. Good correlation between IL-17, IL-6, 
      and IL 1beta suggest that either they are co-regulated or they regulate each other.
FAU - Singh, Amit Kumar
AU  - Singh AK
AD  - Department of Immunology, Sanjay Gandhi Postgraduate Institute of Medical 
      Sciences, Lucknow, India.
FAU - Misra, Ramnath
AU  - Misra R
FAU - Aggarwal, Amita
AU  - Aggarwal A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101222
PL  - Germany
TA  - Clin Rheumatol
JT  - Clinical rheumatology
JID - 8211469
RN  - 0 (Interleukin-17)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-23)
RN  - 0 (Interleukin-6)
RN  - 0 (Interleukins)
RN  - 0 (PHB2 protein, human)
RN  - 0 (Prohibitins)
RN  - 0 (Transforming Growth Factor beta)
RN  - MKM3CA6LT1 (interleukin-21)
SB  - IM
MH  - Adult
MH  - Arthritis, Reactive/*metabolism
MH  - Arthritis, Rheumatoid/metabolism
MH  - Case-Control Studies
MH  - Female
MH  - Humans
MH  - Interleukin-17/*metabolism
MH  - Interleukin-1beta/*metabolism
MH  - Interleukin-23/metabolism
MH  - Interleukin-6/*metabolism
MH  - Interleukins/metabolism
MH  - Male
MH  - Osteoarthritis/metabolism
MH  - Prohibitins
MH  - Retrospective Studies
MH  - Spondylarthropathies/*metabolism
MH  - Synovial Fluid/*metabolism
MH  - Synovitis/metabolism
MH  - Th17 Cells/*metabolism
MH  - Transforming Growth Factor beta/metabolism
EDAT- 2010/12/25 06:00
MHDA- 2011/09/23 06:00
CRDT- 2010/12/25 06:00
PHST- 2010/04/27 00:00 [received]
PHST- 2010/11/30 00:00 [accepted]
PHST- 2010/10/28 00:00 [revised]
PHST- 2010/12/25 06:00 [entrez]
PHST- 2010/12/25 06:00 [pubmed]
PHST- 2011/09/23 06:00 [medline]
AID - 10.1007/s10067-010-1646-5 [doi]
PST - ppublish
SO  - Clin Rheumatol. 2011 Jun;30(6):771-6. doi: 10.1007/s10067-010-1646-5. Epub 2010 
      Dec 22.