PMID- 21183242
OWN - NLM
STAT- MEDLINE
DCOM- 20110902
LR  - 20181201
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 216
IP  - 6
DP  - 2011 Jun
TI  - Dendritic cells matured by a prostaglandin E2-containing cocktail can produce 
      high levels of IL-12p70 and are more mature and Th1-biased than dendritic cells 
      treated with TNF-alpha or LPS.
PG  - 649-62
LID - 10.1016/j.imbio.2010.11.004 [doi]
AB  - Dendritic cells (DCs) play a crucial role in the initiation of an immune 
      response. As maturation is critical for effective antigen presentation, different 
      methods have been used to generate mature DCs (mDCs) ex vivo. The use of a 
      maturation cocktail (MC) consisting of IL-1beta, IL-6, TNF-alpha, and prostaglandin E2 
      (PGE2) initially showed promising results, but then was challenged because of low 
      production of IL-12p70 and the potential for induction of Th2-type immune 
      responses. To investigate this contention, we compared two of the most commonly 
      used maturation factors, TNF-alpha and LPS, with MC. Maturation cocktail was superior 
      to TNF-alpha and LPS with respect to enhancement of mDC-specific surface marker 
      expression (CD83, CD86, and HLA-DR), induction of T cell proliferation by mDCs, 
      and directional motility of mDCs toward CCL19. These results were supported by 
      increased expression of a significant number of additional maturation-related 
      genes by MC in comparison to TNF-alpha and LPS. In addition, we did not observe a 
      Th2-biased shift in the gene expression profile of mDCs generated by MC. 
      Conversely, MC induced more Th1-promoting transcriptional changes than LPS or 
      TNF-alpha, including increased transcript levels of Th1-type cytokines such as IL-15, 
      IL-12beta, and EBI3 (IL-27beta) and MHC class I molecules, Th1-promoting changes in the 
      transcripts of CXCL16, CCL13, and CCL18, and reduced transcript levels of MHC 
      class II molecules. More interestingly, the Th1-promoting characteristics of 
      MC-mDCs were confirmed by their potential to produce large amounts of IL-12p70 
      after effective stimulation simulating in vivo events.
CI  - Copyright (c) 2010 Elsevier GmbH. All rights reserved.
FAU - Landi, Abdolamir
AU  - Landi A
AD  - Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 
      Veterinary Rd, Saskatoon, SK S7N5E3, Canada.
FAU - Babiuk, Lorne A
AU  - Babiuk LA
FAU - van Drunen Littel-van den Hurk, Sylvia
AU  - van Drunen Littel-van den Hurk S
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101125
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 187348-17-0 (Interleukin-12)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Adult
MH  - Cells, Cultured
MH  - Culture Techniques/*methods
MH  - Dendritic Cells/cytology/*drug effects/*immunology
MH  - Dinoprostone/*pharmacology
MH  - Female
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/drug effects/immunology
MH  - Humans
MH  - Interleukin-12/metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - Male
MH  - Th1 Cells/cytology/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Young Adult
EDAT- 2010/12/25 06:00
MHDA- 2011/09/03 06:00
CRDT- 2010/12/25 06:00
PHST- 2010/07/06 00:00 [received]
PHST- 2010/11/11 00:00 [revised]
PHST- 2010/11/17 00:00 [accepted]
PHST- 2010/12/25 06:00 [entrez]
PHST- 2010/12/25 06:00 [pubmed]
PHST- 2011/09/03 06:00 [medline]
AID - S0171-2985(10)00215-9 [pii]
AID - 10.1016/j.imbio.2010.11.004 [doi]
PST - ppublish
SO  - Immunobiology. 2011 Jun;216(6):649-62. doi: 10.1016/j.imbio.2010.11.004. Epub 
      2010 Nov 25.