PMID- 21184213
OWN - NLM
STAT- MEDLINE
DCOM- 20110505
LR  - 20220310
IS  - 2190-3883 (Electronic)
IS  - 1234-1983 (Linking)
VI  - 52
IP  - 1
DP  - 2011 Feb
TI  - A new long terminal repeat (LTR) sequence allows to identify J genome from J S 
      and St genomes of Thinopyrum intermedium.
PG  - 31-3
LID - 10.1007/s13353-010-0019-8 [doi]
AB  - A repetitive sequence of 491 bp, named pMD232-500, was isolated from S. cereale 
      cv. Kustro using wheat SSR marker Xgwm232. GenBank BLAST search revealed that the 
      sequence of pMD232-500 was highly similar to a part of retrotransposon Nusif-1. 
      Fluorescence in situ hybridization (FISH) analysis using pMD232-500 as probe 
      indicated that only 14 Thinopyrum intermedium chromosomes and all the chromosomes 
      of S. cereale cv. Kustro bear FISH signals, however, no FISH signals were 
      observed on Dasypyrum villosum chromosomes. In addition, the FISH signals were 
      distributed on whole arms except their terminal regions. Further genomic in situ 
      hybridization (GISH) analysis using genomic DNA from Pseudoroegneria spicata 
      indicated that the 14 Th. intermedium chromosomes bearing FISH signals should 
      belong to J genome. Thereafter, the repetitive elements pMD232-500 showed the 
      unambiguous features of genomic constitution of Th. intermedium. In addition, the 
      results in the present study have indicated the similarity of genomes from Th. 
      intermedium and S. cereale.
FAU - Tang, Z X
AU  - Tang ZX
AD  - State Key Laboratory of Plant Breeding and Genetics, Sichuan Agriculture 
      University, Ya'an, Sichuan, 625014, China.
FAU - Yang, Z J
AU  - Yang ZJ
FAU - Fu, S L
AU  - Fu SL
FAU - Yang, M Y
AU  - Yang MY
FAU - Li, G R
AU  - Li GR
FAU - Zhang, H Q
AU  - Zhang HQ
FAU - Tan, F Q
AU  - Tan FQ
FAU - Ren, Zhenglong
AU  - Ren Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101224
PL  - England
TA  - J Appl Genet
JT  - Journal of applied genetics
JID - 9514582
SB  - IM
MH  - Chromosomes, Plant
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Nucleic Acid Hybridization
MH  - Poaceae/*classification/*genetics
MH  - Terminal Repeat Sequences/*genetics
MH  - Triticum/*genetics
EDAT- 2010/12/25 06:00
MHDA- 2011/05/06 06:00
CRDT- 2010/12/25 06:00
PHST- 2010/05/23 00:00 [received]
PHST- 2010/07/14 00:00 [accepted]
PHST- 2010/06/21 00:00 [revised]
PHST- 2010/12/25 06:00 [entrez]
PHST- 2010/12/25 06:00 [pubmed]
PHST- 2011/05/06 06:00 [medline]
AID - 10.1007/s13353-010-0019-8 [doi]
PST - ppublish
SO  - J Appl Genet. 2011 Feb;52(1):31-3. doi: 10.1007/s13353-010-0019-8. Epub 2010 Dec 
      24.