PMID- 21185167
OWN - NLM
STAT- MEDLINE
DCOM- 20110504
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 26
IP  - 6
DP  - 2011 Feb 15
TI  - Nanocrystalline diamond impedimetric aptasensor for the label-free detection of 
      human IgE.
PG  - 2987-93
LID - 10.1016/j.bios.2010.11.053 [doi]
AB  - Like antibodies, aptamers are highly valuable as bioreceptor molecules for 
      protein biomarkers because of their excellent selectivity, specificity and 
      stability. The integration of aptamers with semiconducting materials offers great 
      potential for the development of reliable aptasensors. In this paper we present 
      an aptamer-based impedimetric biosensor using a nanocrystalline diamond (NCD) 
      film as a working electrode for the direct and label-free detection of human 
      immunoglobulin E (IgE). Amino (NH(2))-terminated IgE aptamers were covalently 
      attached to carboxyl (COOH)-modified NCD surfaces using carbodiimide chemistry. 
      Electrochemical impedance spectroscopy (EIS) was applied to measure the changes 
      in interfacial electrical properties that arise when the aptamer-functionalized 
      diamond surface was exposed to IgE solutions. During incubation, the formation of 
      aptamer-IgE complexes caused a significant change in the capacitance of the 
      double-layer, in good correspondence with the IgE concentration. The linear 
      dynamic range of IgE detection was from 0.03 mug/mL to 42.8 mug/mL. The detection 
      limit of the aptasensor reached physiologically relevant concentrations (0.03 
      mug/mL). The NCD-based aptasensor was demonstrated to be highly selective even in 
      the presence of a large excess of IgG. In addition, the aptasensor provided 
      reproducible signals during six regeneration cycles. The impedimetric aptasensor 
      was successfully tested on human serum samples, which opens up the potential of 
      using EIS for direct and label-free detection of IgE levels in blood serum.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Tran, Dinh T
AU  - Tran DT
AD  - BIOSYST-MeBioS, Katholieke Universiteit Leuven, Willem de Croylaan 42, B-3001 
      Leuven, Belgium. thidinh.tran@biw.kuleuven.be
FAU - Vermeeren, Veronique
AU  - Vermeeren V
FAU - Grieten, Lars
AU  - Grieten L
FAU - Wenmackers, Sylvia
AU  - Wenmackers S
FAU - Wagner, Patrick
AU  - Wagner P
FAU - Pollet, Jeroen
AU  - Pollet J
FAU - Janssen, Kris P F
AU  - Janssen KP
FAU - Michiels, Luc
AU  - Michiels L
FAU - Lammertyn, Jeroen
AU  - Lammertyn J
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101205
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Aptamers, Nucleotide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 7782-40-3 (Diamond)
SB  - IM
MH  - *Aptamers, Nucleotide/genetics
MH  - Base Sequence
MH  - Biosensing Techniques/instrumentation/*methods/statistics & numerical data
MH  - Diamond
MH  - Dielectric Spectroscopy
MH  - Electrochemical Techniques
MH  - Equipment Design
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - *Nanoparticles
MH  - Nanotechnology
EDAT- 2010/12/28 06:00
MHDA- 2011/05/05 06:00
CRDT- 2010/12/28 06:00
PHST- 2010/09/17 00:00 [received]
PHST- 2010/11/19 00:00 [revised]
PHST- 2010/11/30 00:00 [accepted]
PHST- 2010/12/28 06:00 [entrez]
PHST- 2010/12/28 06:00 [pubmed]
PHST- 2011/05/05 06:00 [medline]
AID - S0956-5663(10)00819-5 [pii]
AID - 10.1016/j.bios.2010.11.053 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2011 Feb 15;26(6):2987-93. doi: 10.1016/j.bios.2010.11.053. 
      Epub 2010 Dec 5.