PMID- 21185437
OWN - NLM
STAT- MEDLINE
DCOM- 20110407
LR  - 20101227
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 486
DP  - 2011
TI  - Determining the distribution of marine and coastal ammonia-oxidizing archaea and 
      bacteria using a quantitative approach.
PG  - 205-21
LID - 10.1016/B978-0-12-381294-0.00009-2 [doi]
AB  - The oxidation of ammonia to nitrite is the first and often rate-limiting step in 
      nitrification and plays an important role in both nitrogen and carbon cycling. 
      This process is carried out by two distinct groups of microorganisms: 
      ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). This 
      chapter describes methods for measuring the abundance of AOA and AOB using 
      ammonia monooxygenase subunit A (amoA) genes, with a particular emphasis on 
      marine and coastal systems. We also describe quantitative measures designed to 
      target two specific clades of marine AOA: the "shallow" (group A) and "deep" 
      (group B) water column AOA.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Mosier, Annika C
AU  - Mosier AC
AD  - Department of Environmental Earth System Science, Stanford University, Stanford, 
      California, USA.
FAU - Francis, Christopher A
AU  - Francis CA
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Archaea/*classification/genetics
MH  - Betaproteobacteria/*classification/genetics
MH  - DNA, Archaeal/genetics/isolation & purification
MH  - DNA, Bacterial/genetics/isolation & purification
MH  - Nitrates/metabolism
MH  - Nitrification
MH  - Nitrites/metabolism
MH  - Oxidation-Reduction
MH  - Oxidoreductases/metabolism
MH  - Phylogeny
MH  - Polymerase Chain Reaction
MH  - Seawater/*microbiology
MH  - Sequence Analysis
EDAT- 2010/12/28 06:00
MHDA- 2011/04/08 06:00
CRDT- 2010/12/28 06:00
PHST- 2010/12/28 06:00 [entrez]
PHST- 2010/12/28 06:00 [pubmed]
PHST- 2011/04/08 06:00 [medline]
AID - B978-0-12-381294-0.00009-2 [pii]
AID - 10.1016/B978-0-12-381294-0.00009-2 [doi]
PST - ppublish
SO  - Methods Enzymol. 2011;486:205-21. doi: 10.1016/B978-0-12-381294-0.00009-2.