PMID- 21186060
OWN - NLM
STAT- MEDLINE
DCOM- 20110218
LR  - 20110124
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 48
IP  - 5
DP  - 2011 Feb
TI  - Two Litopenaeus vannamei HMGB proteins interact with transcription factors LvSTAT 
      and LvDorsal to activate the promoter of white spot syndrome virus 
      immediate-early gene ie1.
PG  - 793-9
LID - 10.1016/j.molimm.2010.11.020 [doi]
AB  - White spot syndrome virus (WSSV) has caused great economic damage to shrimp 
      aquaculture. Previous studies have shown that WSSV successfully usurps the 
      immunity system of the host for its own gene regulation. To investigate the role 
      of shrimp high mobility group box (HMGB) proteins in WSSV gene regulation, two 
      Litopenaeus vannamei HMGB genes, LvHMGBa and LvHMGBb, were isolated by rapid 
      amplification of cDNA ends (RACE). Recombinant LvHMGBa/b proteins were present in 
      the nucleus of transfected Drosophila Schneider 2 (S2) cells. Luciferase reporter 
      assays revealed that LvHMGBa/b upregulated the WSSV immediate-early (IE) gene 
      (ie1) in a NF-kappaB and STAT binding site-dependent manner. GST pull-down assays 
      demonstrated that LvHMGBa/b interacted with L. vannamei Dorsal (LvDorsal) and L. 
      vannamei STAT (LvSTAT), respectively. LvHMGBa was highly expressed in 
      hepatopancreas while HMGBb was highly expressed in stomach, intestine, heart, 
      antennal gland, and epidermis. Moreover, an immune challenge assay demonstrated 
      that the expression of LvHMGBa/b was upregulated by WSSV infection and that both 
      mRNAs reached peak values at 24 h post-infection. To our knowledge, this is the 
      first report that invertebrate HMGB proteins participates in viral gene 
      regulation.
CI  - Copyright A(c) 2010 Elsevier Ltd. All rights reserved.
FAU - Chen, Yi-Hong
AU  - Chen YH
AD  - MOE Key Laboratory of Aquatic Product Safety/State Key Laboratory for Biocontrol, 
      School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 
      510275, PR China.
FAU - Jia, Xiao-Ting
AU  - Jia XT
FAU - Huang, Xian-De
AU  - Huang XD
FAU - Zhang, Shuang
AU  - Zhang S
FAU - Li, Mei
AU  - Li M
FAU - Xie, Jun-Feng
AU  - Xie JF
FAU - Weng, Shao-Ping
AU  - Weng SP
FAU - He, Jian-Guo
AU  - He JG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101224
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (HMGB Proteins)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (STAT Transcription Factors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Nucleus/metabolism
MH  - Cloning, Molecular
MH  - HMGB Proteins/chemistry/genetics/*metabolism
MH  - Humans
MH  - Immediate-Early Proteins/*genetics
MH  - Penaeidae/*metabolism
MH  - Phylogeny
MH  - *Promoter Regions, Genetic
MH  - Protein Binding
MH  - STAT Transcription Factors/*metabolism
MH  - Transcription Factors/*metabolism
MH  - White spot syndrome virus 1/*genetics
EDAT- 2010/12/28 06:00
MHDA- 2011/02/22 06:00
CRDT- 2010/12/28 06:00
PHST- 2010/09/10 00:00 [received]
PHST- 2010/10/04 00:00 [revised]
PHST- 2010/11/28 00:00 [accepted]
PHST- 2010/12/28 06:00 [entrez]
PHST- 2010/12/28 06:00 [pubmed]
PHST- 2011/02/22 06:00 [medline]
AID - S0161-5890(10)00655-3 [pii]
AID - 10.1016/j.molimm.2010.11.020 [doi]
PST - ppublish
SO  - Mol Immunol. 2011 Feb;48(5):793-9. doi: 10.1016/j.molimm.2010.11.020. Epub 2010 
      Dec 24.