PMID- 21187575
OWN - NLM
STAT- MEDLINE
DCOM- 20110411
LR  - 20171116
IS  - 1875-8622 (Electronic)
IS  - 1386-0291 (Linking)
VI  - 46
IP  - 4
DP  - 2010
TI  - Biorheological changes of dendritic cells at the different differentiation 
      stages.
PG  - 265-73
LID - 10.3233/CH-2010-1334 [doi]
AB  - The differentiation, maturation and functioning of Dendritic cells (DCs) are 
      dynamic processes. This study investigated the changes of DCs' migration ability 
      and biorheological properties during their differentiation. Transmigration assay 
      showed that, DCs' migration rate was improved significantly as they differentiate 
      (p < 0.05); NSC (Rac1 blocker) treatment could significantly decrease their 
      migration rates (p < 0.05). Confocal images showed that, F-actin uniformly 
      distributed in monocytes; with DC's differentiation, F-actin began to remodel and 
      gather at the site of dendrites; the images presented surface ruffles and uneven 
      sawtooth-like cytoskeletal structures. Fluorescence polarization analysis showed 
      that, membrane fluidity was increased significantly with DC's differentiation (p 
      < 0.05). CD62L was upregulated significantly (p < 0.05) on the third and ninth 
      days. CD2 was upregulated significantly (p < 0.05) until the seventh day. DC's 
      electrophoretic mobility was increased continuously, especially increased 
      significantly from the third day to the fifth day and the final stage (p < 0.05). 
      These results indicate that there are significant changes in the biorheological 
      properties of DCs during their differentiation.
FAU - Wang, Xianwei
AU  - Wang X
AD  - Health Science Center, Department of Biophysics, Peking University, Beijing, 
      China.
FAU - Chen, Dan
AU  - Chen D
FAU - Zhang, Xiaolan
AU  - Zhang X
FAU - Jia, Binbin
AU  - Jia B
FAU - Xie, Lide
AU  - Xie L
FAU - Sun, Dagong
AU  - Sun D
FAU - Ka, Weibo
AU  - Ka W
FAU - Yao, Weijuan
AU  - Yao W
FAU - Wen, Zong-Yao
AU  - Wen ZY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Hemorheol Microcirc
JT  - Clinical hemorheology and microcirculation
JID - 9709206
RN  - 0 (Actins)
RN  - 0 (CD2 Antigens)
RN  - 126880-86-2 (L-Selectin)
SB  - IM
MH  - Actins/metabolism
MH  - CD2 Antigens/biosynthesis
MH  - Cell Differentiation/*physiology
MH  - Cell Movement/*physiology
MH  - Dendritic Cells/cytology/*physiology
MH  - Endothelium, Vascular/cytology
MH  - Humans
MH  - L-Selectin/biosynthesis
MH  - Membrane Fluidity
MH  - Microscopy, Confocal
MH  - Monocytes/cytology
EDAT- 2010/12/29 06:00
MHDA- 2011/04/13 06:00
CRDT- 2010/12/29 06:00
PHST- 2010/12/29 06:00 [entrez]
PHST- 2010/12/29 06:00 [pubmed]
PHST- 2011/04/13 06:00 [medline]
AID - M9262714X444744P [pii]
AID - 10.3233/CH-2010-1334 [doi]
PST - ppublish
SO  - Clin Hemorheol Microcirc. 2010;46(4):265-73. doi: 10.3233/CH-2010-1334.