PMID- 21188518
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111110
LR  - 20211020
IS  - 0920-9069 (Print)
IS  - 1573-0778 (Electronic)
IS  - 0920-9069 (Linking)
VI  - 63
IP  - 1
DP  - 2011 Jan
TI  - Long-term cultures of stem/progenitor cells from lobular and ductal breast 
      carcinomas under non-adherent conditions.
PG  - 67-80
LID - 10.1007/s10616-010-9328-3 [doi]
AB  - A small subpopulation of stem/progenitor cells can give rise to the diversity of 
      differentiated cells that comprise the bulk of the tumor. Are proliferating 
      cells, within the bulk of tumor, few cells with uncommon features? The cell 
      biological approach provides a limitless model for studying the hierarchical 
      organization of progenitor subpopulation and identifying potential therapeutic 
      targets. Aim of the study was to expand patients' breast cancer cells for 
      evaluating functional cell properties, and to characterize the protein expression 
      profile of selected cells to be compared with that of primary tumors. Breast 
      cancer cells from estrogen receptor (ERalpha) positive, HER2 negative lobular (LoBS 
      cells) and ductal (DuBS cells) histotype were cultured under non-adherent 
      conditions to form mammospheres. Sorting of the cells by their surface expression 
      of CD24 and CD44 gave rise to subpopulations which were propagated, enriched and 
      characterized for the expression of epithelial and stromal markers. We found that 
      non-adherent culture conditions generate mammospheres of slowly proliferating 
      cells; single cells, dissociated from mammospheres, grow in soft agar; long-term 
      cultured LoBS and DuBS cells, CD44+/CD24low, express cytokeratin 5 (CK5), 
      alpha-smooth muscle actin (alpha-sma) and vimentin, known as markers of 
      basal/myoepithelial cells; and ERalpha (only DuBS cells), HER1 (EGF-Receptor), 
      activated HER2, and cyclinD1 as markers of luminal epithelial cell. Isolates of 
      cells from breast cancer patients may be a tool for a marker-driven testing of 
      targeted therapies.
FAU - Nardone, Agostina
AU  - Nardone A
AD  - Dipartimento di Biologia e Patologia Cellulare e Molecolare "L. Califano", 
      Universita di Napoli Federico II, via Pansini 5, 80131, Naples, Italy.
FAU - Corvigno, Sara
AU  - Corvigno S
FAU - Brescia, Annalisa
AU  - Brescia A
FAU - D'Andrea, Daniel
AU  - D'Andrea D
FAU - Limite, Gennaro
AU  - Limite G
FAU - Veneziani, Bianca Maria
AU  - Veneziani BM
LA  - eng
PT  - Journal Article
DEP - 20101228
PL  - United States
TA  - Cytotechnology
JT  - Cytotechnology
JID - 8807027
PMC - PMC3021146
EDAT- 2010/12/29 06:00
MHDA- 2010/12/29 06:01
PMCR- 2012/01/01
CRDT- 2010/12/29 06:00
PHST- 2010/09/06 00:00 [received]
PHST- 2010/12/06 00:00 [accepted]
PHST- 2010/12/29 06:00 [entrez]
PHST- 2010/12/29 06:00 [pubmed]
PHST- 2010/12/29 06:01 [medline]
PHST- 2012/01/01 00:00 [pmc-release]
AID - 9328 [pii]
AID - 10.1007/s10616-010-9328-3 [doi]
PST - ppublish
SO  - Cytotechnology. 2011 Jan;63(1):67-80. doi: 10.1007/s10616-010-9328-3. Epub 2010 
      Dec 28.