PMID- 21188642
OWN - NLM
STAT- MEDLINE
DCOM- 20110623
LR  - 20211020
IS  - 1861-0293 (Electronic)
IS  - 1340-3443 (Linking)
VI  - 65
IP  - 2
DP  - 2011 Apr
TI  - Simultaneous quantification of marker components in Ojeok-san by HPLC-DAD.
PG  - 375-80
LID - 10.1007/s11418-010-0494-6 [doi]
AB  - A systematic high-performance liquid chromatography-diode array detection 
      (HPLC-DAD) method was developed for the rapid, accurate, and simultaneous 
      quantification of eight marker compounds, paeoniflorin, 6-gingerol, decursin, 
      glycyrrhizin, cinnamic acid, hesperidin, poncirin and magnolol, in Ojeok-san, a 
      traditional Korean herbal medicine. These compounds were separated in less than 
      50 min using a Dionex C(18) column with a gradient elution system of water and 
      methanol at a flow rate of 1 ml/min. All calibration curve of standard components 
      showed excellent linearity (R(2) > 0.9922). Limits of detection (LOD) and limits 
      of quantification (LOQ) ranged from 0.01 to 0.11 mug/ml and 0.03 to 0.34 mug/ml, 
      respectively. The relative standard deviations (RSDs) of data of the intra- and 
      inter-day experiments were less than 2.15 and 2.60%, respectively. The accuracy 
      of recovery test ranged from 94.27 to 107.68% with RSD values 0.15-3.61%. The 
      results of validation showed that the HPLC method was stable and very accurate 
      for the quantification of eight marker components in Ojeok-san.
FAU - Weon, Jin Bae
AU  - Weon JB
AD  - Department of Biomaterials Engineering, Division of Biotechnology and 
      Bioengineering, Kangwon National University, Hyoja-2 Dong, Chuncheon 200-701, 
      Republic of Korea.
FAU - Park, Hwayong
AU  - Park H
FAU - Yang, Hye Jin
AU  - Yang HJ
FAU - Ma, Jin Yeul
AU  - Ma JY
FAU - Ma, Choong Je
AU  - Ma CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101228
PL  - Japan
TA  - J Nat Med
JT  - Journal of natural medicines
JID - 101518405
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - *Medicine, Korean Traditional
MH  - Molecular Structure
MH  - Plant Extracts/*chemistry
EDAT- 2010/12/29 06:00
MHDA- 2011/06/24 06:00
CRDT- 2010/12/29 06:00
PHST- 2010/06/03 00:00 [received]
PHST- 2010/11/15 00:00 [accepted]
PHST- 2010/12/29 06:00 [entrez]
PHST- 2010/12/29 06:00 [pubmed]
PHST- 2011/06/24 06:00 [medline]
AID - 10.1007/s11418-010-0494-6 [doi]
PST - ppublish
SO  - J Nat Med. 2011 Apr;65(2):375-80. doi: 10.1007/s11418-010-0494-6. Epub 2010 Dec 
      28.