PMID- 2118884 OWN - NLM STAT- MEDLINE DCOM- 19901018 LR - 20190510 IS - 0910-5050 (Print) IS - 1876-4673 (Electronic) IS - 0910-5050 (Linking) VI - 81 IP - 8 DP - 1990 Aug TI - Genotypic differentiation of intrahepatically transplanted hyperplastic nodule cells of analbuminemic and normal rat origin by polymerase chain reaction. PG - 711-4 AB - DNA fragments encompassing the region of the seven-base-pair deletion in the albumin gene, which is a characteristic abnormality of Nagase's analbuminemic rat (NAR), were amplified by polymerase chain reaction, and we could differentiate the genotypes of normal rat, homozygous NAR and heterozygous NAR electrophoretically. This genotyping method was applied to the differentiation of hyperplastic nodules on immunostained tissue sections in the intrahepatic transplantation model. When the hyperplastic nodule cells of normal rat were transplanted to the livers of homozygous NAR, the donor cells were effectively differentiated from the host cells by the genotype. FAU - Nishikawa, Y AU - Nishikawa Y AD - Department of Pathology, Asahikawa Medical College. FAU - Sakai, H AU - Sakai H FAU - Inagaki, M AU - Inagaki M FAU - Fukuda, I AU - Fukuda I FAU - Ogawa, K AU - Ogawa K FAU - Nagase, S AU - Nagase S LA - eng PT - Journal Article PL - Japan TA - Jpn J Cancer Res JT - Japanese journal of cancer research : Gann JID - 8509412 RN - 0 (Albumins) SB - IM MH - Albumins/*genetics MH - Animals MH - Base Sequence MH - *Chromosome Deletion MH - *Genotype MH - Heterozygote MH - Homozygote MH - Hyperplasia/chemically induced MH - Liver/analysis/pathology MH - Molecular Sequence Data MH - Polymerase Chain Reaction MH - Rats MH - Rats, Inbred F344 PMC - PMC5918084 EDAT- 1990/08/01 00:00 MHDA- 1990/08/01 00:01 PMCR- 1990/08/01 CRDT- 1990/08/01 00:00 PHST- 1990/08/01 00:00 [pubmed] PHST- 1990/08/01 00:01 [medline] PHST- 1990/08/01 00:00 [entrez] PHST- 1990/08/01 00:00 [pmc-release] AID - CAE711 [pii] AID - 10.1111/j.1349-7006.1990.tb02632.x [doi] PST - ppublish SO - Jpn J Cancer Res. 1990 Aug;81(8):711-4. doi: 10.1111/j.1349-7006.1990.tb02632.x.