PMID- 21189025
OWN - NLM
STAT- MEDLINE
DCOM- 20110607
LR  - 20110215
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Linking)
VI  - 12
IP  - 2
DP  - 2011 Feb 14
TI  - Three-dimensional hierarchical composite scaffolds consisting of 
      polycaprolactone, beta-tricalcium phosphate, and collagen nanofibers: fabrication, 
      physical properties, and in vitro cell activity for bone tissue regeneration.
PG  - 502-10
LID - 10.1021/bm1013052 [doi]
AB  - beta-Tricalcium phosphate (beta-TCP) and collagen have been widely used to regenerate 
      various hard tissues, but although Bioceramics and collagen have various 
      biological advantages with respect to cellular activity, their usage has been 
      limited due to beta-TCP's inherent brittleness and low mechanical properties, along 
      with the low shape-ability of the three-dimensional collagen. To overcome these 
      material deficiencies, we fabricated a new hierarchical scaffold that consisted 
      of a melt-plotted polycaprolactone (PCL)/beta-TCP composite and embedded collagen 
      nanofibers. The fabrication process was combined with general melt-plotting 
      methods and electrospinning. To evaluate the capability of this hierarchical 
      scaffold to act as a biomaterial for bone tissue regeneration, physical and 
      biological assessments were performed. Scanning electron microscope (SEM) 
      micrographs of the fabricated scaffolds indicated that the beta-TCP particles were 
      uniformly embedded in PCL struts and that electrospun collagen nanofibers 
      (diameter = 160 nm) were well layered between the composite struts. By 
      accommodating the beta-TCP and collagen nanofibers, the hierarchical composite 
      scaffolds showed dramatic water-absorption ability (100% increase), increased 
      hydrophilic properties (20%), and good mechanical properties similar to PCL/beta-TCP 
      composite. MTT assay and SEM images of cell-seeded scaffolds showed that the 
      initial attachment of osteoblast-like cells (MG63) in the hierarchical scaffold 
      was 2.2 times higher than that on the PCL/beta-TCP composite scaffold. Additionally, 
      the proliferation rate of the cells was about two times higher than that of the 
      composite scaffold after 7 days of cell culture. Based on these results, we 
      conclude that the collagen nanofibers and beta-TCP particles in the scaffold provide 
      good synergistic effects for cell activity.
FAU - Yeo, MyungGu
AU  - Yeo M
AD  - Department of Mechanical Engineering, Chosun University, Gwangju, Korea.
FAU - Lee, Hyeongjin
AU  - Lee H
FAU - Kim, GeunHyung
AU  - Kim G
LA  - eng
PT  - Journal Article
DEP - 20101228
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Calcium Phosphates)
RN  - 0 (Polyesters)
RN  - 0 (beta-tricalcium phosphate)
RN  - 24980-41-4 (polycaprolactone)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - *Bone Regeneration
MH  - Calcium Phosphates/*chemistry/metabolism
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Collagen/*chemistry/metabolism
MH  - Humans
MH  - Nanofibers/*chemistry
MH  - Osteoblasts/chemistry/cytology/*metabolism
MH  - Particle Size
MH  - Polyesters/*chemistry/metabolism
EDAT- 2010/12/30 06:00
MHDA- 2011/06/08 06:00
CRDT- 2010/12/30 06:00
PHST- 2010/12/30 06:00 [entrez]
PHST- 2010/12/30 06:00 [pubmed]
PHST- 2011/06/08 06:00 [medline]
AID - 10.1021/bm1013052 [doi]
PST - ppublish
SO  - Biomacromolecules. 2011 Feb 14;12(2):502-10. doi: 10.1021/bm1013052. Epub 2010 
      Dec 28.