PMID- 21190122
OWN - NLM
STAT- MEDLINE
DCOM- 20120710
LR  - 20211020
IS  - 1420-908X (Electronic)
IS  - 1023-3830 (Linking)
VI  - 60
IP  - 6
DP  - 2011 Jun
TI  - Effects of endogenous substance P expression on degranulation in RBL-2H3 cells.
PG  - 541-6
LID - 10.1007/s00011-010-0301-6 [doi]
AB  - OBJECTIVE AND DESIGN: To determine whether RBL-2H3 cells have endogenous 
      substance P (SP) expression under immunoglobulin E (IgE)-activated and 
      inactivated conditions, and to ascertain the function of endogenous SP in the 
      antigen-induced degranulation of RBL-2H3 cells. MATERIALS AND METHODS: SP mRNA 
      and protein expression in both inactivated and 2,4-dinitrophenol (DNP)-specific 
      IgE-activated RBL-2H3 cells were assessed by real-time PCR and 
      immunofluorescence, respectively. Following activation with DNP-specific IgE, the 
      degranulation of RBL-2H3 cells in response to DNP-bovine serum albumin (BSA), 
      with and without endogenous SP expression, was assessed by monitoring the release 
      of the granular enzyme beta-hexosaminidase. RESULTS: Endogenous SP mRNA and peptide 
      expression increased in activated RBL-2H3 cells, compared with inactivated 
      RBL-2H3 cells. The small hairpin RNA (shRNA)-mediated knockdown of endogenous SP 
      reduced the degranulation ability of RBL-2H3 cells. CONCLUSIONS: Activated 
      RBL-2H3 cells express endogenous SP which plays a role in antigen-induced 
      degranulation.
FAU - Hu, Hua
AU  - Hu H
AD  - Department of ENT, Huadong Hospital, Shanghai Medical School, Fudan University, 
      Shanghai, 200032, People's Republic of China.
FAU - Zhang, Ruxin
AU  - Zhang R
FAU - Fang, Xiaoyun
AU  - Fang X
FAU - Yu, Min
AU  - Yu M
FAU - Yu, Shaoqing
AU  - Yu S
FAU - Zhang, Jie
AU  - Zhang J
FAU - Wang, Hong
AU  - Wang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101229
PL  - Switzerland
TA  - Inflamm Res
JT  - Inflammation research : official journal of the European Histamine Research 
      Society ... [et al.]
JID - 9508160
RN  - 0 (Dinitrophenols)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (dinitrophenyl-bovine serum albumin)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 33507-63-0 (Substance P)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - *Cell Degranulation/drug effects
MH  - Cell Line, Tumor
MH  - Cerebral Cortex/immunology
MH  - Dinitrophenols/pharmacology
MH  - Gene Knockdown Techniques
MH  - Immunoglobulin E/immunology
MH  - Mast Cells/drug effects/*immunology
MH  - RNA, Messenger/metabolism
MH  - RNA, Small Interfering/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serum Albumin, Bovine/pharmacology
MH  - Substance P/genetics/*immunology
EDAT- 2010/12/31 06:00
MHDA- 2012/07/11 06:00
CRDT- 2010/12/31 06:00
PHST- 2010/05/07 00:00 [received]
PHST- 2010/12/14 00:00 [accepted]
PHST- 2010/11/02 00:00 [revised]
PHST- 2010/12/31 06:00 [entrez]
PHST- 2010/12/31 06:00 [pubmed]
PHST- 2012/07/11 06:00 [medline]
AID - 10.1007/s00011-010-0301-6 [doi]
PST - ppublish
SO  - Inflamm Res. 2011 Jun;60(6):541-6. doi: 10.1007/s00011-010-0301-6. Epub 2010 Dec 
      29.