PMID- 21190445
OWN - NLM
STAT- MEDLINE
DCOM- 20110927
LR  - 20171116
IS  - 1557-9077 (Electronic)
IS  - 1050-7256 (Linking)
VI  - 21
IP  - 5
DP  - 2011 May
TI  - Thyroid hormones influence human dendritic cells' phenotype, function, and 
      subsets distribution.
PG  - 533-40
LID - 10.1089/thy.2010.0183 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are the most effective antigen-presenting cells 
      and key regulators of immune response. The immunoregulatory properties of DCs 
      strongly depend on the microenvironment in which DCs have been matured and 
      activated. Thyroid hormones are an important part of this environment and 
      regulate many vital processes including growth and cellular metabolism. The aim 
      of the study was an analysis of the influence of thyroid hormones on blood DC 
      subtypes ex vivo, including the surface expression of molecules involved in 
      antigen presentation, costimulation, and maturation, as well as on functional 
      properties of DCs in vitro. METHODS: Blood samples for the quantitative and 
      phenotypic analysis of peripheral blood plasmacytoid and myeloid DC subtypes were 
      collected from thyroidectomized patients at two time points: (i) at the time of 
      the so-called stimulation with endogenous thyrotropin-a group of hypothyroid 
      patients after l-thyroxine (L-T(4)) withdrawal (pretreatment group)-and (ii) 
      after 2 months of L-T(4) administration for thyrotropin suppression-a 
      posttreatment group. The phenotype of DCs including HLA-DR, costimulatory 
      molecules (CD40, CD80, and CD86), and maturation marker CD83 was assessed by flow 
      cytometry. The influence of isolated peripheral blood DCs on autologous 
      peripheral blood mononuclear cell proliferation and cytokine secretion 
      (interferon alpha, interleukin-12) under triiodothyronine (T(3)) deficiency or 
      T(3) excess was investigated in culture experiments. RESULTS: The percentage of 
      peripheral blood plasmacytoid and myeloid DCs was higher after L-T(4) 
      administration when compared with the pretreatment group. Moreover, the 
      expression of CD86 on both DC subtypes was higher in the L-T(4) treated than in 
      the hypothyroid patients. In the in vitro experiments, T(3) stimulation increased 
      CD86 expression on cultured DCs. The phenotypic difference was paralleled by 
      enhanced ability of T(3)-stimulated DCs to activate interleukin-12 secretion and 
      proliferation of autologous peripheral blood mononuclear cells (PBMLs) in 
      coculture experiments. CONCLUSIONS: In the present study, we provide for the 
      first time an evidence that the thyrometabolic status has an influence on the 
      phenotype and function of human peripheral blood DCs. This observation may be of 
      potential importance for the understanding of the pathogenesis of immune and 
      endocrine disorders.
FAU - Dedecjus, Marek
AU  - Dedecjus M
AD  - Department of General, Oncological, and Endocrine Surgery, Polish Mother's 
      Memorial Hospital-Research Institute, Medical University of Lodz, Lodz, Poland. 
      mdedecjus@wp.pl
FAU - Stasiolek, Mariusz
AU  - Stasiolek M
FAU - Brzezinski, Jan
AU  - Brzezinski J
FAU - Selmaj, Krzysztof
AU  - Selmaj K
FAU - Lewinski, Andrzej
AU  - Lewinski A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101229
PL  - United States
TA  - Thyroid
JT  - Thyroid : official journal of the American Thyroid Association
JID - 9104317
RN  - 0 (B7-2 Antigen)
RN  - 0 (Cytokines)
RN  - 0 (Thyroid Hormones)
RN  - 9002-71-5 (Thyrotropin)
RN  - Q51BO43MG4 (Thyroxine)
SB  - IM
MH  - Adult
MH  - B7-2 Antigen/biosynthesis
MH  - Case-Control Studies
MH  - Cell Separation
MH  - Cytokines/metabolism
MH  - Dendritic Cells/metabolism/*pathology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Hypothyroidism/metabolism/pathology
MH  - Lymphocyte Subsets
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - Thyroid Gland/physiology
MH  - Thyroid Hormones/*metabolism
MH  - Thyrotropin/metabolism
MH  - Thyroxine/metabolism
EDAT- 2010/12/31 06:00
MHDA- 2011/09/29 06:00
CRDT- 2010/12/31 06:00
PHST- 2010/12/31 06:00 [entrez]
PHST- 2010/12/31 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
AID - 10.1089/thy.2010.0183 [doi]
PST - ppublish
SO  - Thyroid. 2011 May;21(5):533-40. doi: 10.1089/thy.2010.0183. Epub 2010 Dec 29.