PMID- 21192913
OWN - NLM
STAT- MEDLINE
DCOM- 20110620
LR  - 20220318
IS  - 1096-0309 (Electronic)
IS  - 0003-2697 (Linking)
VI  - 411
IP  - 2
DP  - 2011 Apr 15
TI  - Quantitative analysis of tissue folate using ultra high-performance liquid 
      chromatography tandem mass spectrometry.
PG  - 210-7
LID - 10.1016/j.ab.2010.12.033 [doi]
AB  - The tissue distribution of folate in its numerous coenzyme forms may influence 
      the development of disease at different sites. For instance, the susceptibility 
      of human colonic mucosa to localized folate deficiency may predispose to the 
      development of colorectal cancer. We report a sensitive and robust ultra 
      high-performance liquid chromatography (UHPLC) tandem mass spectrometry method 
      for quantifying tissue H(4)folate, 5-CH(3)-H(4)folate, 5-CHO-H(4)folate, folic 
      acid, and 5,10-CH(+)-H(4)folate concentration. Human colonic mucosa (20-100mg) 
      was extracted using lipase and conjugase enzyme digestion. Rapid separation of 
      analytes was achieved on a UHPLC 1.9-mum C18 column over 7 min. Accurate 
      quantitation was performed using stable isotopically labeled ((13)C(5)) internal 
      standards. The instrument response was linear over physiological concentrations 
      of tissue folate (R(2)>0.99). Limits of detection and quantitation were less than 
      20 and 30 fmol on column, respectively, and within- and between-run imprecision 
      values were 6-16%. In colonic mucosal samples from 73 individuals, the average 
      molar distribution of folate coenzymes was 58% 5-CH(3)-H(4)folate, 20% 
      H(4)folate, 18% formyl-H(4)folate (sum of 5-CHO-H(4)folate and 
      5,10-CH(+)-H(4)folate), and 4% folic acid. This assay would be useful in 
      characterizing folate distribution in human and animal tissues as well as the 
      role of deregulated folate homeostasis on disease pathogenesis.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Liu, Jia
AU  - Liu J
AD  - Prince of Wales Clinical School and Adult Cancer Program, Lowy Cancer Research 
      Centre, University of New South Wales, Sydney, NSW 2052, Australia.
FAU - Pickford, Russell
AU  - Pickford R
FAU - Meagher, Alan P
AU  - Meagher AP
FAU - Ward, Robyn L
AU  - Ward RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101228
PL  - United States
TA  - Anal Biochem
JT  - Analytical biochemistry
JID - 0370535
RN  - 935E97BOY8 (Folic Acid)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Colorectal Neoplasms/metabolism
MH  - Folic Acid/*analysis
MH  - Humans
MH  - Intestinal Mucosa/metabolism
MH  - Liver/metabolism
MH  - Mice
MH  - Rats
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2011/01/05 06:00
MHDA- 2011/06/21 06:00
CRDT- 2011/01/04 06:00
PHST- 2010/09/25 00:00 [received]
PHST- 2010/12/19 00:00 [revised]
PHST- 2010/12/21 00:00 [accepted]
PHST- 2011/01/04 06:00 [entrez]
PHST- 2011/01/05 06:00 [pubmed]
PHST- 2011/06/21 06:00 [medline]
AID - S0003-2697(10)00820-1 [pii]
AID - 10.1016/j.ab.2010.12.033 [doi]
PST - ppublish
SO  - Anal Biochem. 2011 Apr 15;411(2):210-7. doi: 10.1016/j.ab.2010.12.033. Epub 2010 
      Dec 28.