PMID- 21192983
OWN - NLM
STAT- MEDLINE
DCOM- 20110919
LR  - 20181201
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 172
IP  - 1-2
DP  - 2011 Mar
TI  - A multiplex RT-PCR for rapid and simultaneous detection of porcine teschovirus, 
      classical swine fever virus, and porcine reproductive and respiratory syndrome 
      virus in clinical specimens.
PG  - 88-92
LID - 10.1016/j.jviromet.2010.12.023 [doi]
AB  - A multiplex RT-PCR (mRT-PCR) assay was developed and evaluated for its ability to 
      detect multiple viral infections of swine simultaneously. One pair of primers was 
      selected carefully for each of the following three RNA viruses: porcine 
      reproductive and respiratory syndrome virus (PRRSV), classical swine fever virus 
      (CSFV), and porcine teschovirus (PTV). Each target produced a specific amplicon 
      with a size of 451bp (PRRSV), 343bp (CSFV), or 163bp (PTV). The sensitivity of 
      the mRT-PCR using purified plasmid constructs containing the specific viral 
      target fragments was 2.02 x 10(2), 2.90 x 10(3), and 6.16 x 10(3) copies for PRRSV, 
      CSFV, and PTV, respectively. Among 69 clinical samples from Heilongjiang, Jilin, 
      and Henan provinces, co-infection by PRRSV and CSFV was 4.4%, co-infection by 
      PRRSV and PTV was 11.6%, co-infection by PTV and CSFV was 13.0%, and co-infection 
      by the three viruses was 8.7%. In conclusion, the mRT-PCR should be useful for 
      routine molecular diagnosis and epidemiology.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Liu, Shanshan
AU  - Liu S
AD  - Division of Swine Infectious Diseases, State Key Laboratory of Veterinary 
      Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of 
      Agricultural Sciences, No. 427, Maduan Street, Nangang District, Harbin 150001, 
      China.
FAU - Zhao, Yarong
AU  - Zhao Y
FAU - Hu, Quanbo
AU  - Hu Q
FAU - Lv, Chaochao
AU  - Lv C
FAU - Zhang, Chaofan
AU  - Zhang C
FAU - Zhao, Rui
AU  - Zhao R
FAU - Hu, Feng
AU  - Hu F
FAU - Lin, Wencheng
AU  - Lin W
FAU - Cui, Shangjin
AU  - Cui S
LA  - eng
PT  - Journal Article
DEP - 20101228
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Classical Swine Fever Virus/genetics/physiology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/physiology
MH  - RNA Virus Infections/diagnosis/*veterinary
MH  - RNA Viruses/genetics/*physiology
MH  - RNA, Viral
MH  - *Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/*diagnosis/*virology
MH  - Teschovirus/genetics/physiology
EDAT- 2011/01/05 06:00
MHDA- 2011/09/20 06:00
CRDT- 2011/01/04 06:00
PHST- 2010/11/03 00:00 [received]
PHST- 2010/12/11 00:00 [revised]
PHST- 2010/12/21 00:00 [accepted]
PHST- 2011/01/04 06:00 [entrez]
PHST- 2011/01/05 06:00 [pubmed]
PHST- 2011/09/20 06:00 [medline]
AID - S0166-0934(10)00464-7 [pii]
AID - 10.1016/j.jviromet.2010.12.023 [doi]
PST - ppublish
SO  - J Virol Methods. 2011 Mar;172(1-2):88-92. doi: 10.1016/j.jviromet.2010.12.023. 
      Epub 2010 Dec 28.