PMID- 21193919
OWN - NLM
STAT- MEDLINE
DCOM- 20110809
LR  - 20211020
IS  - 1434-4726 (Electronic)
IS  - 0937-4477 (Linking)
VI  - 268
IP  - 5
DP  - 2011 May
TI  - Transcriptional activities of histone H3, cyclin D1 and claudin 7 encoding genes 
      in laryngeal cancer.
PG  - 709-14
LID - 10.1007/s00405-010-1471-0 [doi]
AB  - Uncontrolled proliferation and a decrease in cell-cell adhesion are one of the 
      most important characteristics of malignancy. Determination of 
      replication-dependent histone H3 can be applied as a proliferative marker. Cyclin 
      D1 (CCND1) regulates the cell cycle by participating in the control of the G1/S 
      phase transition. Claudins (CLDN) are components of tight junctions and may play 
      an essential role in the loss of tissue cohesion. The aim of the study was to 
      assess the mRNA expression of histone H3, cyclin D1, and claudin 7 genes in 
      laryngeal squamous cell carcinoma (LSCC) and adjacent nonneoplastic tissues. The 
      study group consisted of 32 patients with LSCC. Adjacent nonneoplastic tissues of 
      incision lines were used as controls. Quantification of H3, CCND1 and CLDN7 mRNAs 
      was performed by the use of real-time QRT-PCR assay. Molecular analysis showed a 
      significantly higher expression of CCND1 (P = 0.0001; Wilcoxon test) and H3 (P = 
      0.0141) genes in tumor tissues than in surrounding nonneoplastic tissues. On the 
      contrary, transcriptional activity of claudin 7 gene was higher in histologically 
      normal tissues; however, this difference was not statistically significant (P = 
      0.1499). The data obtained indicate that laryngeal cancer is characterized by 
      high proliferative potential mediated by increase in cyclin D1 and H3 mRNAs 
      expression.
CI  - (c) Springer-Verlag 2010
FAU - Kapral, Malgorzata
AU  - Kapral M
AD  - Department of Biochemistry, Medical University of Silesia, Narcyzow 1, 41-200, 
      Sosnowiec, Poland. mkapral@sum.edu.pl
FAU - Strzalka-Mrozik, Barbara
AU  - Strzalka-Mrozik B
FAU - Kowalczyk, Malgorzata
AU  - Kowalczyk M
FAU - Paluch, Jaroslaw
AU  - Paluch J
FAU - Gola, Joanna
AU  - Gola J
FAU - Gierek, Tatiana
AU  - Gierek T
FAU - Weglarz, Ludmila
AU  - Weglarz L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101231
PL  - Germany
TA  - Eur Arch Otorhinolaryngol
JT  - European archives of oto-rhino-laryngology : official journal of the European 
      Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the 
      German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
JID - 9002937
RN  - 0 (CLDN7 protein, human)
RN  - 0 (Claudins)
RN  - 0 (Histones)
RN  - 0 (Membrane Proteins)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/pathology
MH  - Cell Cycle/genetics
MH  - Cell Proliferation
MH  - Claudins
MH  - Cyclin D1/*genetics/metabolism
MH  - Female
MH  - Histones/*genetics/metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - Male
MH  - Membrane Proteins/*genetics/metabolism
MH  - Middle Aged
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tight Junctions/genetics
MH  - Transcriptional Activation/*genetics
EDAT- 2011/01/05 06:00
MHDA- 2011/08/10 06:00
CRDT- 2011/01/04 06:00
PHST- 2010/06/08 00:00 [received]
PHST- 2010/12/16 00:00 [accepted]
PHST- 2011/01/04 06:00 [entrez]
PHST- 2011/01/05 06:00 [pubmed]
PHST- 2011/08/10 06:00 [medline]
AID - 10.1007/s00405-010-1471-0 [doi]
PST - ppublish
SO  - Eur Arch Otorhinolaryngol. 2011 May;268(5):709-14. doi: 
      10.1007/s00405-010-1471-0. Epub 2010 Dec 31.