PMID- 21196763
OWN - NLM
STAT- MEDLINE
DCOM- 20110729
LR  - 20110517
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 155
IP  - 2
DP  - 2011
TI  - Arginine kinase from the cellar spider (Holocnemus pluchei): a new asthma-causing 
      allergen.
PG  - 180-6
LID - 10.1159/000319822 [doi]
AB  - BACKGROUND: We report a 31-year-old farmer whose work consists in handling cereal 
      and vegetables, who consulted our clinic because of asthma symptoms after 
      inhalation of dust during manipulation of the deposited material, usually inside 
      the warehouse. METHODS AND RESULTS: Skin prick tests and specific immunoglobulin 
      E (IgE) determinations were negative with common aeroallergens. The patient noted 
      the presence of many spiders in the warehouse, which were identified as the 
      cellar spider Holocnemus pluchei and the common house spider, Tegenaria 
      domestica. Extracts of spider bodies brought in by the patient were obtained and 
      used to perform in vivo and in vitro studies. Molecular characterization of 
      IgE-binding bands was performed by mass spectrometry. We obtained positive prick 
      tests to the extracts of the bodies of both spiders. Immunoblotting displayed 
      different bands in both spider extracts, in a range of 20-70 kDa. All were 
      hemocyanins, except for a 17-kDa protein of Holocnemus identified as an arginine 
      kinase (AK). Bronchial challenge was positive with the extract of the cellar 
      spider and with the AK, but was negative with the domestic house spider. 
      CONCLUSION: We present the first case of respiratory allergy due to sensitization 
      to AK from a common spider, confirmed by bronchial provocation tests.
CI  - Copyright (c) 2010 S. Karger AG, Basel.
FAU - Bobolea, Irina
AU  - Bobolea I
AD  - Allergy Department, Hospital Universitario La Paz-IdiPAZ, Madrid, Spain.
FAU - Barranco, Pilar
AU  - Barranco P
FAU - Pastor-Vargas, Carlos
AU  - Pastor-Vargas C
FAU - Iraola, Victor
AU  - Iraola V
FAU - Vivanco, Fernando
AU  - Vivanco F
FAU - Quirce, Santiago
AU  - Quirce S
LA  - eng
PT  - Case Reports
PT  - Journal Article
DEP - 20101223
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Allergens)
RN  - 0 (Cell Extracts)
RN  - 0 (Galectin 3)
RN  - EC 2.7.3.3 (Arginine Kinase)
SB  - IM
MH  - Adult
MH  - Allergens/analysis/immunology
MH  - Animals
MH  - Arginine Kinase/*adverse effects/analysis/immunology
MH  - Asthma/*diagnosis/*etiology/physiopathology
MH  - Bronchial Provocation Tests
MH  - Cell Extracts/chemistry/immunology
MH  - Environmental Exposure/*adverse effects
MH  - Galectin 3/analysis/immunology
MH  - Humans
MH  - *Immunization
MH  - Male
MH  - Mass Spectrometry
MH  - Spiders/immunology
EDAT- 2011/01/05 06:00
MHDA- 2011/07/30 06:00
CRDT- 2011/01/04 06:00
PHST- 2010/03/02 00:00 [received]
PHST- 2010/07/21 00:00 [accepted]
PHST- 2011/01/04 06:00 [entrez]
PHST- 2011/01/05 06:00 [pubmed]
PHST- 2011/07/30 06:00 [medline]
AID - 000319822 [pii]
AID - 10.1159/000319822 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2011;155(2):180-6. doi: 10.1159/000319822. Epub 2010 
      Dec 23.