PMID- 21198863
OWN - NLM
STAT- MEDLINE
DCOM- 20110523
LR  - 20191210
IS  - 1600-0609 (Electronic)
IS  - 0902-4441 (Linking)
VI  - 86
IP  - 4
DP  - 2011 Apr
TI  - Determination of platelet-bound glycoprotein-specific autoantibodies by flow 
      cytometric immunobead assay in primary immune thrombocytopenia.
PG  - 339-46
LID - 10.1111/j.1600-0609.2010.01572.x [doi]
AB  - OBJECTIVES: Primary immune thrombocytopenia (ITP) is an autoimmune disorder 
      characterized by premature platelet destruction induced by autoantibodies 
      directed against platelet glycoproteins (GPs). Despite being a clinically 
      important disorder, ITP lacks a feasible diagnostic assay for routine clinical 
      use. This study was meant to evaluate a newly developed flow cytometric 
      immunobead assay for determination of platelet-bound GP-specific autoantibodies 
      in comparison with indirect monoclonal antibody-specific immobilization of 
      platelet antigen (MAIPA) in the diagnosis of ITP. METHODS: Platelet-bound and 
      plasma GPIIb/IIIa and GPIb/IX autoantibodies were determined by flow cytometric 
      immunobead assay and indirect modified MAIPA, respectively. The average 
      fluorescence level for platelet-bound, GP-specific autoantibodies was given as a 
      ratio to three normal controls tested simultaneously. RESULTS: The median value 
      of platelet-bound GPIIb/IIIa and GPIb/IX autoantibodies in ITP group were 3.09 
      (range 0.78, 30.2) and 3.09 (range 0.72, 19.2), respectively, which were 
      significantly higher than non-ITP group [1.01 (0.67, 5.59) and 1.01 (0.79, 5.56), 
      respectively, P<0.001] and normal controls [1.02 (0.72, 1.76) and 1.03 (0.79, 
      1.73), respectively, P<0.001]. The receiver-operating characteristics curve 
      analysis showed an area under the curve of 0.895 for GPIIb/IIIa autoantibody and 
      0.859 for GPIb/IX autoantibody, respectively. Combined detection of GPIIb/IIIa or 
      GPIb/IX autoantibodies by flow cytometric immunobead assay showed a sensitivity 
      of 82.11% for ITP diagnosis. CONCLUSION: This study demonstrated that 
      determination of platelet-bound, GP-specific autoantibodies by flow cytometric 
      immunobead assay was a convenient, sensitive, and specific test for the 
      differential diagnosis of thrombocytopenic patients.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Liu, Xin-Guang
AU  - Liu XG
AD  - Department of Hematology, Qilu Hospital, Shandong University, Jinan, China.
FAU - Li, Jin-Lin
AU  - Li JL
FAU - Qin, Ping
AU  - Qin P
FAU - Ren, Juan
AU  - Ren J
FAU - Ma, Shi-Hui
AU  - Ma SH
FAU - Sun, Lin
AU  - Sun L
FAU - Shi, Yan
AU  - Shi Y
FAU - Ji, Xue-Bin
AU  - Ji XB
FAU - Zhu, Yuan-Yuan
AU  - Zhu YY
FAU - Ma, Dao-Xin
AU  - Ma DX
FAU - Guo, Cheng-Shan
AU  - Guo CS
FAU - Du, Xin
AU  - Du X
FAU - Hou, Ming
AU  - Hou M
FAU - Peng, Jun
AU  - Peng J
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110218
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (Antigens, Human Platelet)
RN  - 0 (Autoantibodies)
RN  - 0 (Platelet Membrane Glycoproteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antibody Specificity
MH  - Antigens, Human Platelet/blood
MH  - Autoantibodies/*blood
MH  - Blood Platelets/*immunology
MH  - Case-Control Studies
MH  - Female
MH  - Flow Cytometry/methods
MH  - Humans
MH  - Immunoassay/*methods
MH  - Male
MH  - Middle Aged
MH  - Platelet Membrane Glycoproteins/immunology
MH  - Purpura, Thrombocytopenic, Idiopathic/*blood/diagnosis/*immunology
MH  - Young Adult
EDAT- 2011/01/05 06:00
MHDA- 2011/05/24 06:00
CRDT- 2011/01/05 06:00
PHST- 2011/01/05 06:00 [entrez]
PHST- 2011/01/05 06:00 [pubmed]
PHST- 2011/05/24 06:00 [medline]
AID - 10.1111/j.1600-0609.2010.01572.x [doi]
PST - ppublish
SO  - Eur J Haematol. 2011 Apr;86(4):339-46. doi: 10.1111/j.1600-0609.2010.01572.x. 
      Epub 2011 Feb 18.