PMID- 21199006
OWN - NLM
STAT- MEDLINE
DCOM- 20110131
LR  - 20231011
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 44
IP  - 1
DP  - 2011 Feb
TI  - Foe turned friend: multiple functional roles attributable to hyper-activating 
      stem cell factor receptor mutant in regeneration of the haematopoietic cell 
      compartment.
PG  - 10-8
LID - 10.1111/j.1365-2184.2010.00713.x [doi]
AB  - OBJECTIVES: Stem cell factor receptor, c-kit, is considered to be the master 
      signalling molecule of haematopoietic stem cells. It develops the orchestral 
      pattern of haematopoietic cell lineages, seen by its varying degree of 
      omnipresence in progenitors, lineage committed and mature cells. We have 
      investigated the effect of over-expressing c-kit on early recovery of the 
      haematopoietic compartment, in irradiated hosts. MATERIALS AND METHODS: Normal 
      bone marrow cells (BMCs) were transfected with Kit(wt) (wild-type c-kit) or its 
      variant Kit(mu) (asp814tyr) by electroporation. Lethally irradiated mice were 
      transplanted with normal or transfected congeneic BMCs. The effect of ectopic 
      expression of c-kit on haematopoietic cell recovery was determined by analysing 
      donor-derived cells. Furthermore, effects of both types of c-kit over-expression 
      on progenitor and lineage-committed cells were examined by flow cytometric 
      analysis of Sca-1 and lineage-committed (Lin(+)) cells respectively. RESULTS: 
      Hyper-activating Kit(mu) significantly improved recovery of the haematopoietic 
      system in irradiated hosts. In vivo results showed that the donor-derived 
      c-kit(+) cell population was increased to more than 3-fold in the case of 
      Kit(mu)-transfected cells compared to normal and Kit(wt) over-expressing BMCs. In 
      general, survival of progenitor and committed cell was improved in the Kit(mu) 
      over-expressing system compared to the other two cohorts. CONCLUSION: These 
      results suggest that recruitment of the hyper-activating variant of c-kit 
      (Kit(mu)) lead to early recovery of the bone marrow of lethally irradiated mice.
CI  - (c) 2010 Blackwell Publishing Ltd.
FAU - Pati, S
AU  - Pati S
AD  - Stem Cell Biology Laboratory, National Institute of Immunology, Aruna Asaf Ali 
      Marg, New Delhi, India.
FAU - Kalra, O P
AU  - Kalra OP
FAU - Mukhopadhyay, A
AU  - Mukhopadhyay A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/chemistry/radiation effects
MH  - Bone Marrow Transplantation
MH  - Cell Lineage
MH  - Electroporation
MH  - Hematopoietic System/*chemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Mutation
MH  - Proto-Oncogene Proteins c-kit/analysis/*genetics/pharmacology
MH  - *Regeneration
PMC - PMC6496452
EDAT- 2011/01/05 06:00
MHDA- 2011/02/01 06:00
PMCR- 2010/12/29
CRDT- 2011/01/05 06:00
PHST- 2011/01/05 06:00 [entrez]
PHST- 2011/01/05 06:00 [pubmed]
PHST- 2011/02/01 06:00 [medline]
PHST- 2010/12/29 00:00 [pmc-release]
AID - CPR713 [pii]
AID - 10.1111/j.1365-2184.2010.00713.x [doi]
PST - ppublish
SO  - Cell Prolif. 2011 Feb;44(1):10-8. doi: 10.1111/j.1365-2184.2010.00713.x.