PMID- 21204293
OWN - NLM
STAT- MEDLINE
DCOM- 20110505
LR  - 20191210
IS  - 1942-7611 (Electronic)
IS  - 1942-7603 (Linking)
VI  - 2
IP  - 11-12
DP  - 2010 Nov-Dec
TI  - Degradation of urine samples and its influence on the (1)(3)C/(1)(2)C ratios of excreted 
      steroids.
PG  - 620-9
LID - 10.1002/dta.219 [doi]
AB  - The degradation processes in deficiently stored urine samples are well 
      investigated regarding steroid concentrations and diagnostic ratios, such as the 
      quotient of testosterone divided by epitestosterone. In contrast, nothing is 
      known about the influence on carbon isotope ratios (CIR) by inappropriate storage 
      conditions. In general, it is assumed that degradation, i.e. deconjugation or 
      dehydrogenation, does not change CIR and thus CIR can be used in cases where the 
      steroid profile turns out to be invalid. Therefore, the CIR of urinary steroids 
      was investigated in different urine samples during the course of degradation over 
      a time period of six months. Several steroids excreted as glucuronides 
      (androsterone (A), etiocholanolone (E), testosterone, pregnanediol (PD) and 5alpha- 
      and 5beta-androstane-3alpha,17beta-diol) or sulfo-conjugated (A, E and 
      androst-5-ene-3beta,17beta-diol (5EN17b)) were investigated together with their 
      unconjugated correspondents (A, E, PD and 5EN17b) and the main dehydrogenation 
      products (5alpha- and 5beta-androstane-3,17-dion and androst-4-ene-3,17-dion). For this 
      purpose, the exiting methods for CIR determination were extended and validated. 
      In addition, the urinary concentrations of all investigated steroids were 
      monitored. Particular attention was paid to dehydroepiandrosterone conjugated and 
      unconjugated together with its degradation product 
      3alpha,5-cyclo-5alpha-androstan-6beta-ol-17-one as here the strongest influence on CIR was 
      expected.
CI  - Copyright (c) 2010 John Wiley & Sons, Ltd.
FAU - Piper, Thomas
AU  - Piper T
AD  - Institute of Biochemistry, German Sport University Cologne, Am Sportpark 
      Mungersdorf 6, Koln, Germany. t.piper@biochem.dshs-koeln.de
FAU - Geyer, Hans
AU  - Geyer H
FAU - Schanzer, Wilhelm
AU  - Schanzer W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20101116
PL  - England
TA  - Drug Test Anal
JT  - Drug testing and analysis
JID - 101483449
RN  - 0 (Carbon Isotopes)
RN  - 0 (Glucuronides)
RN  - 0 (Steroids)
RN  - 3XMK78S47O (Testosterone)
RN  - 481-30-1 (Epitestosterone)
SB  - IM
MH  - Carbon Isotopes/urine
MH  - Doping in Sports
MH  - Epitestosterone/metabolism/urine
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Glucuronides/metabolism/urine
MH  - Humans
MH  - Male
MH  - *Specimen Handling
MH  - Steroids/metabolism/*urine
MH  - Substance Abuse Detection/*methods
MH  - Testosterone/metabolism/urine
EDAT- 2011/01/05 06:00
MHDA- 2011/05/06 06:00
CRDT- 2011/01/05 06:00
PHST- 2010/07/01 00:00 [received]
PHST- 2010/09/24 00:00 [revised]
PHST- 2010/10/02 00:00 [accepted]
PHST- 2011/01/05 06:00 [entrez]
PHST- 2011/01/05 06:00 [pubmed]
PHST- 2011/05/06 06:00 [medline]
AID - 10.1002/dta.219 [doi]
PST - ppublish
SO  - Drug Test Anal. 2010 Nov-Dec;2(11-12):620-9. doi: 10.1002/dta.219. Epub 2010 Nov 
      16.