PMID- 21204876
OWN - NLM
STAT- MEDLINE
DCOM- 20110418
LR  - 20181201
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 52
IP  - 3
DP  - 2011 Mar
TI  - Optimization of bioassay for tetracycline detection in milk by means of 
      chemometric techniques.
PG  - 245-52
LID - 10.1111/j.1472-765X.2010.02990.x [doi]
AB  - AIMS: In this study, a microbiological method of dichotomous response using 
      Bacillus cereus was designed and optimized to detect tetracyclines (TCs) at 
      concentrations near to the maximum residue limits (MRLs). METHODS AND RESULTS: In 
      a first stage, the response time of bioassay was reduced to 5 h when the 
      logarithm of spore concentration (log S) was increased. Later, a Plackett Burman 
      design (2(6-3)) was analysed using logistic regression model. This design 
      indicates significant effects of log S and chloramphenicol (CAP) on the detection 
      limit (DL) of TC. Then, the response surfaces (RS) of the TCs DTs as a function 
      of log S and CAP were plotted using a Dohlert design and the logistic regression 
      model. These RS show a linear decrease with the raise of CAP and a quadratic 
      effect of log S. Finally, the DTs of TC (109 mug l(-1)) and oxytetracycline (100 
      mug l(-1)) were adjusted to their MRLs through the desirability function. 
      CONCLUSIONS: By successive application of experimental design techniques could be 
      optimized a bioassay for the detection of TC residues in milk. The best 
      conditions have been achieved when the assay was made with log S = 5.12 and CAP = 
      470 mug l(-1). SIGNIFICANCE AND IMPACT OF THE STUDY: Experimental design 
      techniques together with the logistic regression model and the desirability 
      function represent an adequate tool for the optimization of a bioassay with 
      binary response.
CI  - (c) 2011 The Authors. Letters in Applied Microbiology (c) 2011 The Society for 
      Applied Microbiology.
FAU - Nagel, O G
AU  - Nagel OG
AD  - Catedra de Biofisica, Departamento de Ciencias Basicas, Facultad de Ciencias 
      Veterinarias, Universidad Nacional del Litoral, Esperanza, Argentina.
FAU - Molina, M P
AU  - Molina MP
FAU - Althaus, R L
AU  - Althaus RL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110124
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Tetracyclines)
RN  - 66974FR9Q1 (Chloramphenicol)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Bacillus cereus/drug effects
MH  - Biological Assay/*methods
MH  - Chloramphenicol/chemistry
MH  - Food Analysis/*methods
MH  - Limit of Detection
MH  - Logistic Models
MH  - Microbial Sensitivity Tests
MH  - Milk/*chemistry
MH  - Sensitivity and Specificity
MH  - Spores, Bacterial/drug effects
MH  - Tetracyclines/*analysis
EDAT- 2011/01/06 06:00
MHDA- 2011/04/19 06:00
CRDT- 2011/01/06 06:00
PHST- 2011/01/06 06:00 [entrez]
PHST- 2011/01/06 06:00 [pubmed]
PHST- 2011/04/19 06:00 [medline]
AID - 10.1111/j.1472-765X.2010.02990.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2011 Mar;52(3):245-52. doi: 
      10.1111/j.1472-765X.2010.02990.x. Epub 2011 Jan 24.