PMID- 21204880
OWN - NLM
STAT- MEDLINE
DCOM- 20110418
LR  - 20181201
IS  - 1472-765X (Electronic)
IS  - 0266-8254 (Linking)
VI  - 52
IP  - 3
DP  - 2011 Mar
TI  - FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of 
      different hosts.
PG  - 220-6
LID - 10.1111/j.1472-765X.2010.02994.x [doi]
AB  - AIMS: To investigate the spatial organization of endogenous and exogenously 
      applied Lactobacillus communities at specific locations in the adult 
      gastrointestinal tract of different hosts. METHODS AND RESULTS: Samples of the 
      human, murine and avian gastrointestinal tract of subjects that received or not 
      received a Lactobacillus probiotic were analysed by fluorescence in situ 
      hybridization (FISH) with rRNA-targeted probes. High levels of endogenous 
      lactobacilli were observed on the nonsecretory, stratified squamous epithelia 
      present in the forestomach of mice and crop of chickens, respectively. These 
      epithelial associations showed characteristics of bacterial biofilms, i.e. 
      bacteria attached to a surface and embedded in a matrix of extracellular 
      polymeric substances. In other regions of the analysed intestines, lactobacilli 
      seemed to occur mainly as dispersed bacterial cells or as microcolonies. 
      Exogenous administration of a Lactobacillus probiotic did increase the levels of 
      loosely adherent Lactobacillus cells detected. However, the probiotic strains 
      were unable to establish themselves inside the gastrointestinal biofilms. 
      CONCLUSIONS: Gastrointestinal biofilms of lactobacilli occur only in specific 
      niches in certain hosts, such as the murine forestomach and avian crop. 
      SIGNIFICANCE AND IMPACT OF THE STUDY: Biofilm formation by lactobacilli in 
      specific parts of animal gastrointestinal tracts was documented for the first 
      time by FISH.
CI  - (c) 2011 The Authors. Letters in Applied Microbiology (c) 2011 The Society for 
      Applied Microbiology.
FAU - Lebeer, S
AU  - Lebeer S
AD  - Centre of Microbial and Plant Genetics, K.U.Leuven, Leuven, Belgium. 
      sarah.lebeer@biw.kuleuven.be
FAU - Verhoeven, T L A
AU  - Verhoeven TL
FAU - Claes, I J J
AU  - Claes IJ
FAU - De Hertogh, G
AU  - De Hertogh G
FAU - Vermeire, S
AU  - Vermeire S
FAU - Buyse, J
AU  - Buyse J
FAU - Van Immerseel, F
AU  - Van Immerseel F
FAU - Vanderleyden, J
AU  - Vanderleyden J
FAU - De Keersmaecker, S C J
AU  - De Keersmaecker SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lett Appl Microbiol
JT  - Letters in applied microbiology
JID - 8510094
SB  - IM
MH  - Animals
MH  - *Biofilms
MH  - Chickens
MH  - Crop, Avian/microbiology
MH  - Female
MH  - Gastrointestinal Tract/*microbiology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Intestines/microbiology
MH  - Lactobacillus/genetics/*growth & development
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Probiotics
MH  - Randomized Controlled Trials as Topic
MH  - Stomach/microbiology
EDAT- 2011/01/06 06:00
MHDA- 2011/04/19 06:00
CRDT- 2011/01/06 06:00
PHST- 2011/01/06 06:00 [entrez]
PHST- 2011/01/06 06:00 [pubmed]
PHST- 2011/04/19 06:00 [medline]
AID - 10.1111/j.1472-765X.2010.02994.x [doi]
PST - ppublish
SO  - Lett Appl Microbiol. 2011 Mar;52(3):220-6. doi: 10.1111/j.1472-765X.2010.02994.x.