PMID- 21208279
OWN - NLM
STAT- MEDLINE
DCOM- 20110202
LR  - 20191210
IS  - 1600-0463 (Electronic)
IS  - 0903-4641 (Linking)
VI  - 119
IP  - 2
DP  - 2011 Feb
TI  - C3b-induced eosinophil degranulation involves PI3-kinases and is inhibited by 
      protein kinase C activity.
PG  - 119-26
LID - 10.1111/j.1600-0463.2010.02701.x [doi]
AB  - Selective release of individual eosinophil granule proteins has been demonstrated 
      in eosinophilic conditions and in vitro using different stimuli. The aim of this 
      study was to investigate if selective release of eosinophil cationic protein 
      (ECP), eosinophil protein X/eosinophil derived-neurotoxin (EPX/EDN) and 
      eosinophil peroxidase (EPO) could be due to the involvement of different signal 
      transduction pathways. Peripheral blood granulocytes from healthy donors were 
      incubated with Wortmannin, LY294002, Genistein, Staurosporine, GO6976 or PD98059 
      prior to the induction of degranulation by C3b. The released amounts of ECP, EPO 
      and EPX/EDN were determined by immunoassays, and related to the total cell 
      content of respective protein. Wortmannin caused a significant, dose-dependent 
      inhibition of all three granule proteins. LY294002 (10(-)(6) M) also inhibited the 
      release of all proteins. Genistein (10(-)(6) M) inhibited the release of ECP, whereas 
      the release of EPO was increased. However, there was a tendency towards similar 
      concentration-dependent patterns of release of all three proteins. Staurosporine 
      (10(-)(7) M), GO6976 (10(-)(6) M) and PD98059 (10(-)(5) M) caused an increased release of the 
      three proteins. PI3-kinases play an important role in the C3b-induced release of 
      ECP, EPO and EPX/EDN, whereas protein kinase C seems to have inhibitory effects 
      on C3b-induced degranulation.
CI  - (c) 2010 The Authors. APMIS (c) 2010 APMIS.
FAU - Carlson, Marie
AU  - Carlson M
AD  - Department of Medical Sciences, University Hospital, Uppsala University, Sweden. 
      marie.carlson@akademiska.se
FAU - Venge, Per
AU  - Venge P
FAU - Lampinen, Maria
AU  - Lampinen M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101201
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (Carbazoles)
RN  - 0 (Flavonoids)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 136194-77-9 (Go 6976)
RN  - 80295-43-8 (Complement C3b)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - H88EPA0A3N (Staurosporine)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Carbazoles/pharmacology
MH  - *Cell Degranulation
MH  - Complement C3b/*physiology
MH  - Eosinophils/*physiology
MH  - Extracellular Signal-Regulated MAP Kinases/physiology
MH  - Flavonoids/pharmacology
MH  - Humans
MH  - Phosphatidylinositol 3-Kinases/*physiology
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Protein Kinase C/*physiology
MH  - Protein Kinase Inhibitors/pharmacology
MH  - Staurosporine/pharmacology
EDAT- 2011/01/07 06:00
MHDA- 2011/02/03 06:00
CRDT- 2011/01/07 06:00
PHST- 2011/01/07 06:00 [entrez]
PHST- 2011/01/07 06:00 [pubmed]
PHST- 2011/02/03 06:00 [medline]
AID - 10.1111/j.1600-0463.2010.02701.x [doi]
PST - ppublish
SO  - APMIS. 2011 Feb;119(2):119-26. doi: 10.1111/j.1600-0463.2010.02701.x. Epub 2010 
      Dec 1.