PMID- 21208534
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110106
IS  - 1009-3419 (Print)
IS  - 1009-3419 (Linking)
VI  - 8
IP  - 6
DP  - 2005 Dec 20
TI  - [Optimizing the methods of whole lung cancer RNA-loaded dendritic cells].
PG  - 489-94
LID - 10.3779/j.issn.1009-3419.2005.06.01 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are the unique antigen-presenting cells that 
      can activate naive T lymphocytes. This function is critical for inducing specific 
      immune response. DCs-based vaccines have been used broadly in immunotherapy for 
      many carcinomas. Constructing vaccines by transfecting total tumor RNA into DCs 
      can be done with a few tumor tissues and need not to identify tumor antigens, so 
      it is especially suitable for lung cancer which lacks tumor-specific antigens but 
      has great heterogenicity and weak immunogenicity. Currently, the best 
      transfection stage and method are still indefinite. So, the objective of this 
      study is to explore the best condition of transfecting total RNA extracted from 
      lung cancer tissues into DCs. METHODS: Ten patients with lung cancer were 
      enrolled whose tumor tissues were CEA and MUC1 positive in immunohistochemical 
      staining. Total tumor RNA were extracted by one-step method. Then DCs and T cells 
      were separated and cultured from peripheral blood monocytes and the RNA was 
      transfected into the DCs in different stages with different methods. CEA and MUC1 
      expression in the transfected DCs were measured by flow cytometry analysis and T 
      cells' proliferation was examined by mixed lymphocyte reaction (MLR). RESULTS: 
      The expression of CEA and MUC1 protein in immature DCs (11.33+/-2.64, 39.68+/-7.25) 
      was remarkably higher than that in mature DCs (5.46+/-1.63, 27.17+/-4.16) after 
      transfection with total RNA of lung cancer tissues (P < 0.01), and the DCs 
      presented more powerful effects on T cell proliferation. The CEA and MUC1 
      expression on DCs were significantly higher in electroporation transfection group 
      (20.53+/-3.64, 65.39+/- 9.33) than that in lipofection group (11.33+/-2.64, 39.68+/-7.25) 
      and passive pulsing transfection group ( 0.91+/-0.27,18.53+/-3.26)(P < 0.01), and the 
      DCs in electroporation transfection group presented more powerful effects on 
      stimulating T cell proliferation than the other two groups did. CONCLUSIONS: 
      Transfecting total tumor RNA into immature DCs by using electroporation is a good 
      way to construct DCs-based vaccines for lung cancer and to achieve a higher 
      activity to stimulate T cell proliferation.
FAU - Wang, Kun
AU  - Wang K
AD  - Cancer Center, Guangdong Provincial People's Hospital, Guangdong Provincial Lung 
      Cancer Research Institute, Guangzhou, Guangdong 510080, P.R.China.
FAU - Wu, Yilong
AU  - Wu Y
FAU - Zhou, Qing
AU  - Zhou Q
FAU - Xu, Chongrui
AU  - Xu C
FAU - Lin, Jiaying
AU  - Lin J
FAU - Yang, Xuening
AU  - Yang X
FAU - Huang, Shaoqiong
AU  - Huang S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Fei Ai Za Zhi
JT  - Zhongguo fei ai za zhi = Chinese journal of lung cancer
JID - 101126433
EDAT- 2005/12/20 00:00
MHDA- 2005/12/20 00:01
CRDT- 2011/01/07 06:00
PHST- 2011/01/07 06:00 [entrez]
PHST- 2005/12/20 00:00 [pubmed]
PHST- 2005/12/20 00:01 [medline]
AID - 10.3779/j.issn.1009-3419.2005.06.01 [doi]
PST - ppublish
SO  - Zhongguo Fei Ai Za Zhi. 2005 Dec 20;8(6):489-94. doi: 
      10.3779/j.issn.1009-3419.2005.06.01.