PMID- 21209554
OWN - NLM
STAT- MEDLINE
DCOM- 20110718
LR  - 20211020
IS  - 2092-6413 (Electronic)
IS  - 1226-3613 (Print)
IS  - 1226-3613 (Linking)
VI  - 43
IP  - 2
DP  - 2011 Feb 28
TI  - Epigallocatechin-3-gallate inhibits paracrine and autocrine hepatocyte growth 
      factor/scatter factor-induced tumor cell migration and invasion.
PG  - 111-20
AB  - Aberrant activation of hepatocyte growth factor/scatter factor (HGF/SF) and its 
      receptor, Met, is involved in the development and progression of many human 
      cancers. In the cell-based screening assay, (-)epigallocatechin-3-gallate (EGCG) 
      inhibited HGF/SF-Met signaling as indicated by its inhibitory activity on 
      HGF/SF-induced cell scattering and uPA activation (IC50=15.8 microgram/ml). 
      Further analysis revealed that EGCG at low doses specifically inhibited 
      HGF/SF-induced tyrosine phosphorylation of Met but not epidermal growth factor 
      (EGF)-induced phosphorylation of EGF receptor (EGFR). On the other hand, 
      high-dose EGCG decreased both Met and EGFR proteins. We also found that EGCG did 
      not act on the intracellular portion of Met receptor tyrosine kinase, i.e., it 
      inhibited InlB-dependent activation of Met but not NGF-induced activation of 
      Trk-Met hybrid receptor. This inhibition decreased HGF-induced migration and 
      invasion by parental or HGF/SF-transfected B16F10 melanoma cells in vitro in 
      either a paracrine or autocrine manner. Furthermore, EGCG inhibited the 
      invasion/metastasis of HGF/SF-transfected B16F10 melanoma cells in mice. Our data 
      suggest the possible use of EGCG in human cancers associated with dysregulated 
      paracrine or autocrine HGF/SF-Met signaling.
FAU - Kwak, In-hae
AU  - Kwak IH
AD  - Department of Biochemistry, Ajou University Medical School, Suwon, Korea.
FAU - Shin, Yun-Hye
AU  - Shin YH
FAU - Kim, Myeongdeok
AU  - Kim M
FAU - Cha, Hyun-Young
AU  - Cha HY
FAU - Nam, Hyun-Ja
AU  - Nam HJ
FAU - Lee, Bok-Soon
AU  - Lee BS
FAU - Chaudhary, S C
AU  - Chaudhary SC
FAU - Pai, Ki-Soo
AU  - Pai KS
FAU - Lee, Jae-Ho
AU  - Lee JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Exp Mol Med
JT  - Experimental & molecular medicine
JID - 9607880
RN  - 0 (Receptors, Growth Factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 8R1V1STN48 (Catechin)
RN  - BQM438CTEL (epigallocatechin gallate)
RN  - EC 2.7.10.1 (MET protein, human)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Animals
MH  - Autocrine Communication/*drug effects
MH  - Catechin/*analogs & derivatives/metabolism/pharmacology
MH  - Cell Line, Tumor
MH  - Cell Movement/drug effects
MH  - Female
MH  - *Hepatocyte Growth Factor
MH  - Humans
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neoplasms, Experimental/*metabolism/pathology
MH  - Paracrine Communication/*drug effects
MH  - Phosphorylation/drug effects
MH  - Proto-Oncogene Proteins c-met/antagonists & inhibitors/metabolism
MH  - Receptors, Growth Factor/antagonists & inhibitors/metabolism
MH  - Signal Transduction
PMC - PMC3047192
EDAT- 2011/01/07 06:00
MHDA- 2011/07/19 06:00
PMCR- 2011/02/28
CRDT- 2011/01/07 06:00
PHST- 2011/01/07 06:00 [entrez]
PHST- 2011/01/07 06:00 [pubmed]
PHST- 2011/07/19 06:00 [medline]
PHST- 2011/02/28 00:00 [pmc-release]
AID - emm.2010.43.013 [pii]
AID - 10.3858/emm.2011.43.2.013 [doi]
PST - ppublish
SO  - Exp Mol Med. 2011 Feb 28;43(2):111-20. doi: 10.3858/emm.2011.43.2.013.