PMID- 21211110
OWN - NLM
STAT- MEDLINE
DCOM- 20130906
LR  - 20181202
IS  - 1741-0533 (Electronic)
IS  - 1740-925X (Linking)
VI  - 6
IP  - 3
DP  - 2010 Aug
TI  - Prostaglandin E2 induces glutamate release from subventricular zone astrocytes.
PG  - 201-7
LID - 10.1017/S1740925X10000244 [doi]
AB  - It was recently reported that in one of the adult neurogenetic zones, the 
      subventricular zone (SVZ), astrocyte-like cells release glutamate upon 
      intracellular Ca2+ increases. However, the signals that control Ca2+ activity and 
      glutamate release from SVZ astrocytes are not known. Here, we examined whether 
      prostaglandin E2 (PGE2), which induces glutamate release from mature astrocytes, 
      is such a signal. Using the gramicidin-perforated patch-clamp technique, we show 
      that the activity of N-Methyl-D-Aspartate receptor (NMDAR) channel in neuroblasts 
      is a high fidelity sensor of ambient glutamate levels. Using such sensors, we 
      found that application of PGE2 led to increased ambient glutamate levels in the 
      SVZ. In parallel experiments, PGE2 induced an increase in intracellular Ca2+ 
      levels in SVZ cells, in particular astrocyte-like cells, as shown using Ca2+ 
      imaging. Finally, a PGE2 enzyme immunoassay showed that the choroid plexus of the 
      lateral ventricle and to a lesser extent the SVZ (ten-fold less) released PGE2. 
      These findings suggest that PGE2 is a physiological signal for inducing glutamate 
      release from SVZ astrocytes that is important for controlling neuroblast survival 
      and proliferation. This signal may be accentuated following ischemia or 
      injury-induced PGE2 release and may contribute to the injury-associated increased 
      neurogenesis.
FAU - Dave, Kathleen A
AU  - Dave KA
AD  - Departments of Neurosurgery, and Cellular and Molecular Physiology, Yale 
      University School of Medicine, New Haven, CT 06520-8082, USA.
FAU - Platel, Jean-Claude
AU  - Platel JC
FAU - Huang, Frank
AU  - Huang F
FAU - Tian, David
AU  - Tian D
FAU - Stamboulian-Platel, Severine
AU  - Stamboulian-Platel S
FAU - Bordey, Angelique
AU  - Bordey A
LA  - eng
GR  - DC007681/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110107
PL  - England
TA  - Neuron Glia Biol
JT  - Neuron glia biology
JID - 101217278
RN  - 0 (Receptors, Glutamate)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Astrocytes/*metabolism
MH  - Calcium/metabolism
MH  - Dinoprostone/*metabolism
MH  - Electroporation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glutamic Acid/*metabolism
MH  - Immunohistochemistry
MH  - Mice
MH  - Neural Stem Cells/*cytology/metabolism
MH  - Neurogenesis/*physiology
MH  - Patch-Clamp Techniques
MH  - Receptors, Glutamate/metabolism
MH  - Receptors, N-Methyl-D-Aspartate/metabolism
EDAT- 2011/01/08 06:00
MHDA- 2013/09/07 06:00
CRDT- 2011/01/08 06:00
PHST- 2011/01/08 06:00 [entrez]
PHST- 2011/01/08 06:00 [pubmed]
PHST- 2013/09/07 06:00 [medline]
AID - S1740925X10000244 [pii]
AID - 10.1017/S1740925X10000244 [doi]
PST - ppublish
SO  - Neuron Glia Biol. 2010 Aug;6(3):201-7. doi: 10.1017/S1740925X10000244. Epub 2011 
      Jan 7.