PMID- 21212137
OWN - NLM
STAT- MEDLINE
DCOM- 20110601
LR  - 20211020
IS  - 1944-9917 (Electronic)
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 25
IP  - 3
DP  - 2011 Mar
TI  - A role for nuclear factor interleukin-3 (NFIL3), a critical transcriptional 
      repressor, in down-regulation of periovulatory gene expression.
PG  - 445-59
LID - 10.1210/me.2010-0250 [doi]
AB  - The LH surge triggers dramatic transcriptional changes in genes associated with 
      ovulation and luteinization. The present study investigated the spatiotemporal 
      expression of nuclear factor IL-3 (NFIL3), a transcriptional regulator of the 
      basic leucine zipper transcription factor superfamily, and its potential role in 
      the ovary during the periovulatory period. Immature female rats were injected 
      with pregnant mare's serum gonadotropin, treated with human chorionic 
      gonadotropin (hCG), and ovaries or granulosa cells were collected at various 
      times after hCG. Nfil3 mRNA was highly induced both in intact ovaries and 
      granulosa cells after hCG treatment. In situ hybridization demonstrated that 
      Nfil3 mRNA was highly induced in theca-interstitial cells at 4-8 h after hCG, 
      localized to granulosa cells at 12 h, and decreased at 24 h. Overexpression of 
      NFIL3 in granulosa cells inhibited the induction of prostaglandin-endoperoxide 
      synthase 2 (Ptgs2), progesterone receptor (Pgr), epiregulin (Ereg), and 
      amphiregulin (Areg) and down-regulated levels of prostaglandin E2. The inhibitory 
      effect on Ptgs2 induction was reversed by NFIL3 small interfering RNA treatment. 
      In theca-interstitial cells the expression of hydroxyprostaglandin dehydrogenase 
      15-(nicotinamide adenine dinucleotide) (Hpgd) was also inhibited by NFIL3 
      overexpression. Data from luciferase assays demonstrated that NFIL3 
      overexpression decreased the induction of the Ptgs2 and Areg promoter activity. 
      EMSA and chromatin immunoprecipitation analyses indicated that NFIL3 binds to the 
      promoter region containing the DNA-binding sites of cAMP response element binding 
      protein and CCAAT enhancer binding protein-beta. In summary, hCG induction of NFIL3 
      expression may modulate the process of ovulation and theca-interstitial and 
      granulosa cell differentiation by regulating expression of PTGS2, PGR, AREG, 
      EREG, and HPGD, potentially through interactions with cAMP response element 
      binding protein and CCAAT enhancer binding protein-beta on their target gene 
      promoters.
FAU - Li, Feixue
AU  - Li F
AD  - College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 
      310036, People's Republic of China.
FAU - Liu, Jing
AU  - Liu J
FAU - Jo, Misung
AU  - Jo M
FAU - Curry, Thomas E Jr
AU  - Curry TE Jr
LA  - eng
GR  - R01 HD057446/HD/NICHD NIH HHS/United States
GR  - P20 RR015592/RR/NCRR NIH HHS/United States
GR  - P20 RR 15592/RR/NCRR NIH HHS/United States
GR  - HD051727/HD/NICHD NIH HHS/United States
GR  - HD057446/HD/NICHD NIH HHS/United States
GR  - R03 HD051727/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110106
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (Areg protein, rat)
RN  - 0 (Chorionic Gonadotropin)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (EREG protein, human)
RN  - 0 (Epiregulin)
RN  - 0 (Ereg protein, rat)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (NFIL3 protein, rat)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Repressor Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
SB  - IM
MH  - Amphiregulin
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Chorionic Gonadotropin/pharmacology
MH  - Chromatin Immunoprecipitation
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Down-Regulation/drug effects
MH  - EGF Family of Proteins
MH  - Electrophoretic Mobility Shift Assay
MH  - Epidermal Growth Factor/genetics/metabolism
MH  - Epiregulin
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Glycoproteins/genetics/metabolism
MH  - Granulosa Cells
MH  - Humans
MH  - In Situ Hybridization
MH  - Intercellular Signaling Peptides and Proteins/genetics/metabolism
MH  - Mutagenesis, Site-Directed
MH  - Oligonucleotide Array Sequence Analysis
MH  - Ovary/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Progesterone/genetics/metabolism
MH  - Repressor Proteins/genetics/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC3045738
EDAT- 2011/01/08 06:00
MHDA- 2011/06/02 06:00
PMCR- 2012/03/01
CRDT- 2011/01/08 06:00
PHST- 2011/01/08 06:00 [entrez]
PHST- 2011/01/08 06:00 [pubmed]
PHST- 2011/06/02 06:00 [medline]
PHST- 2012/03/01 00:00 [pmc-release]
AID - me.2010-0250 [pii]
AID - ME-10-0250 [pii]
AID - 10.1210/me.2010-0250 [doi]
PST - ppublish
SO  - Mol Endocrinol. 2011 Mar;25(3):445-59. doi: 10.1210/me.2010-0250. Epub 2011 Jan 
      6.